Formulation and Evaluation of Pluronic F-127 Thermoresponsive Nanogels Containing Juglone for In vivo Wound Healing Potential

Poor solubility, low bioavailability and toxicity of juglone have attracted the attention of the researchers in developing a formulation which can be easily applicable, non-irritant and can accelerate the healing process. Eudragit RS100 based nanoparticles at various concentrations (0.05%, 0.1%, 0.15%) were formulated by solvent evaporation approach providing particle size in the range (89.20–117.1 nm) with PDI values (0.249–0.256) portraying spherical shaped nanoparticles. Highest entrapment efficiency (88.6 ± 0.821%) and drug loading (1.453 ± 0.013%) were observed for juglone nanoparticles of batch having juglone concentration of 0.15%. FTIR analysis confirmed no interaction among the used ingredients while XRD results showed reduced crystallinity of JNPs. In vitro release demonstrated 96.99 ± 6.70% release while antibacterial results illustrated higher inhibitory action of JNP 0.15 batch against the E. coli strain. Pluronic F-127 based gel formulations (JNG 0.05, JNG 0.1, JNG 0.15) was developed for further analysis showing thermoresponsive property. The JNG 0.15 batch showed considerable spreadability (65.17 ± 1.04 mm), in vitro occlusivity (70 ± 3.0%), higher visco-elastic profile (20,197 mPa.s), effective mechanical properties, sustained release effect (72.79 ± 7.20%) and highest inhibitory action against the E. coli strain. Non-irritant and maximum healing ability were observed in batch treated with JNG 0.15 formulation pointing usage of juglone compound for novel future formulation.