Effective solubilization and single-step purification of Bacillus licheniformis [alpha]-amylase from insoluble aggregates

Effective solubilization and single-step purification of Bacillus licheniformis [alpha]-amylase from insoluble aggregates

A high level expression of thermostable α-amylase gene from Bacillus licheniformis in Escherichia coli was obtained. The recombinant enzyme was mainly produced in the form of insoluble aggregates. The enzyme was solubilized without using denaturing agents and purified to homogeneity in a single step...

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Veröffentlicht in:Folia microbiologica 2010-03, Vol.55 (2), p.133
Hauptverfasser: Rashid, N, Ahmed, N, Saleem Haider, M, Haque, I
Format: Artikel
Sprache:eng
Schlagworte:
Aggregates
Bacteria
E coli
Enzymes
Gene expression
Microbiology
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Zusammenfassung:A high level expression of thermostable α-amylase gene from Bacillus licheniformis in Escherichia coli was obtained. The recombinant enzyme was mainly produced in the form of insoluble aggregates. The enzyme was solubilized without using denaturing agents and purified to homogeneity in a single step by ion exchange chromatography. The enzyme was purified 138-fold with a final yield of 349 %; the specific activity of the purified enzyme was 1343 U/mg.[PUBLICATION ABSTRACT]
ISSN:0015-5632
1874-9356
DOI:10.1007/s12223-010-0020-y