TPF stitching imaging of rubber tree leaves

This paper presents a two-photon fluorescence (TPF) microscopy platform based on a femtosecond oscillator. The system images rubber tree leaf samples by exciting and detecting TPF signals generated by the tissue using broadband femtosecond pulses. The imaging system utilizes a detection window of 565–615 nm to capture TPF signals from flavin adenine dinucleotide (FAD) in the rubber tree leaf tissue. Additionally, multiple TPF images were acquired through sample movement and Z-axis scanning, followed by image stitching to achieve large-area comprehensive visualization of the rubber tree leaf samples. This study provides detailed observations and analyses of various biological structures within the rubber tree leaf samples, contributing significantly to the understanding of plant growth, physiological functions, and environmental adaptability.