Nanoparticles formation through photopolymerization of pyrrole with lecithin for enhanced mitochondrial activation via NIR-photothermal mild-hyperthermia

The development of biocompatible nanoparticles (NPs) with effective photothermal properties is essential for advancing biomedical applications. This study investigates the synthesis and characterization of lecithin-polypyrrole nanoparticles (LEC-PPy NPs) formed through near-infrared (NIR) irradiation. LEC-PPy NPs were prepared by mixing lecithin with varying concentrations of pyrrole monomer, followed by NIR irradiation to induce potential polymerization. Characterization techniques, including Dynamic Light Scattering (DLS), Zeta Potential Analysis, FTIR, XRD, and TEM, were employed. The prepared NPs demonstrated a photothermal response, reaching temperatures above around 43 °C under NIR exposure while exhibiting cell viability and minimal hemolysis. The biochemical tests of the cellular membrane potential and AHR further validate their biological response. This study highlights the implication of NIR-induced polymerization in producing biocompatible LEC-PPy NPs, which show potential for various biomedical applications. Further optimization and in vivo validation are necessary to realize their clinical potential.