Establishment, Multiplication, and Biochemical Analysis of Embryogenic Lines of the Amazonian Palm Euterpe precatoria Mart. under Suspension Culture
The palm Euterpe precatoria holds great social, cultural, and environmental importance. The heart of palm and the fruit are the main products used for industrialization due to their energetic properties. Thus, the aim of this study was to establish a suspension cultivation protocol for the species u...
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creator | Vasconcelos, Alexandro Dias Martins Ferreira, Jéssica Cristina Barbosa Meira, Rennan Oliveira Silva-Cardoso, Inaê Mariê de Araújo Neves, Joane dos Santos Souza, Anderson Marcos de Souza, André Luís Xavier de Scherwinski-Pereira, Jonny Everson |
description | The palm Euterpe precatoria holds great social, cultural, and environmental importance. The heart of palm and the fruit are the main products used for industrialization due to their energetic properties. Thus, the aim of this study was to establish a suspension cultivation protocol for the species using different explant sources. For this, eight lineages of E. precatoria embryogenic calluses were tested, with five in liquid medium Murashige and Skoog (MS) with 5 μM Picloram and three for comparison in semisolid medium MS with 20 μM Picloram and 5 μM 2iP. The growth curve was obtained by weighing the calli from 60 to 180 days of cultivation. The Gompertz model was applied, and growth kinetics were evaluated. At 100 days, the contents of total soluble sugars (TSSs) and total soluble proteins (TSPs) were determined. Principal components (PCA) were measured. According to the analysis of the data, the cultivation of E. precatoria lineages in liquid medium was successfully carried out, and the establishment was achieved. The model can be considered adequate since the R2 values found describe more than 90% of the growth kinetics of the lineages. In the liquid system, lineages L1 (from leaf explants and multiplied in semisolid medium—SM), L2 (from leaf explants and multiplied in SM), and L6 (from zygotic embryo explants and multiplied in liquid medium—LM) showed the shortest time to double the biomass accumulation. Multivariate analysis reveals a significant increase in masses in liquid cultures, represented by lineages L6 and L2. There was statistical difference in the amount of TSSs extracted; the highest TSS levels were observed in lineages cultivated in LM. The protein content found was very low, showing statistical differences among the lineages. In this work, the establishment and multiplication of embryogenic calli of E. precatoria are described for the first time, and they emerge as viable alternatives for the vegetative propagation of the species. |
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The heart of palm and the fruit are the main products used for industrialization due to their energetic properties. Thus, the aim of this study was to establish a suspension cultivation protocol for the species using different explant sources. For this, eight lineages of E. precatoria embryogenic calluses were tested, with five in liquid medium Murashige and Skoog (MS) with 5 μM Picloram and three for comparison in semisolid medium MS with 20 μM Picloram and 5 μM 2iP. The growth curve was obtained by weighing the calli from 60 to 180 days of cultivation. The Gompertz model was applied, and growth kinetics were evaluated. At 100 days, the contents of total soluble sugars (TSSs) and total soluble proteins (TSPs) were determined. Principal components (PCA) were measured. According to the analysis of the data, the cultivation of E. precatoria lineages in liquid medium was successfully carried out, and the establishment was achieved. The model can be considered adequate since the R2 values found describe more than 90% of the growth kinetics of the lineages. In the liquid system, lineages L1 (from leaf explants and multiplied in semisolid medium—SM), L2 (from leaf explants and multiplied in SM), and L6 (from zygotic embryo explants and multiplied in liquid medium—LM) showed the shortest time to double the biomass accumulation. Multivariate analysis reveals a significant increase in masses in liquid cultures, represented by lineages L6 and L2. There was statistical difference in the amount of TSSs extracted; the highest TSS levels were observed in lineages cultivated in LM. The protein content found was very low, showing statistical differences among the lineages. In this work, the establishment and multiplication of embryogenic calli of E. precatoria are described for the first time, and they emerge as viable alternatives for the vegetative propagation of the species.</description><identifier>ISSN: 1999-4907</identifier><identifier>EISSN: 1999-4907</identifier><identifier>DOI: 10.3390/f15101804</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Analysis ; Biochemical analysis ; Charcoal ; Cultivation ; Embryos ; Ethylenediaminetetraacetic acid ; Euterpe precatoria ; Explants ; Fruit cultivation ; Growth kinetics ; Kinetics ; Leaves ; Multiplication ; Multivariate analysis ; Picloram ; Plant propagation ; Principal components analysis ; Propagation ; Proteins ; Semisolids ; Statistical analysis ; Sucrose ; Suspension culture</subject><ispartof>Forests, 2024-10, Vol.15 (10), p.1804</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c221t-83fcacefec7adf46a72f669a547852f890661ec4652dee271e953d75e8152a4f3</cites><orcidid>0000-0001-6271-332X ; 0000-0002-5582-5371</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Vasconcelos, Alexandro Dias Martins</creatorcontrib><creatorcontrib>Ferreira, Jéssica Cristina Barbosa</creatorcontrib><creatorcontrib>Meira, Rennan Oliveira</creatorcontrib><creatorcontrib>Silva-Cardoso, Inaê Mariê de Araújo</creatorcontrib><creatorcontrib>Neves, Joane dos Santos</creatorcontrib><creatorcontrib>Souza, Anderson Marcos de</creatorcontrib><creatorcontrib>Souza, André Luís Xavier de</creatorcontrib><creatorcontrib>Scherwinski-Pereira, Jonny Everson</creatorcontrib><title>Establishment, Multiplication, and Biochemical Analysis of Embryogenic Lines of the Amazonian Palm Euterpe precatoria Mart. under Suspension Culture</title><title>Forests</title><description>The palm Euterpe precatoria holds great social, cultural, and environmental importance. The heart of palm and the fruit are the main products used for industrialization due to their energetic properties. Thus, the aim of this study was to establish a suspension cultivation protocol for the species using different explant sources. For this, eight lineages of E. precatoria embryogenic calluses were tested, with five in liquid medium Murashige and Skoog (MS) with 5 μM Picloram and three for comparison in semisolid medium MS with 20 μM Picloram and 5 μM 2iP. The growth curve was obtained by weighing the calli from 60 to 180 days of cultivation. The Gompertz model was applied, and growth kinetics were evaluated. At 100 days, the contents of total soluble sugars (TSSs) and total soluble proteins (TSPs) were determined. Principal components (PCA) were measured. According to the analysis of the data, the cultivation of E. precatoria lineages in liquid medium was successfully carried out, and the establishment was achieved. The model can be considered adequate since the R2 values found describe more than 90% of the growth kinetics of the lineages. In the liquid system, lineages L1 (from leaf explants and multiplied in semisolid medium—SM), L2 (from leaf explants and multiplied in SM), and L6 (from zygotic embryo explants and multiplied in liquid medium—LM) showed the shortest time to double the biomass accumulation. Multivariate analysis reveals a significant increase in masses in liquid cultures, represented by lineages L6 and L2. There was statistical difference in the amount of TSSs extracted; the highest TSS levels were observed in lineages cultivated in LM. The protein content found was very low, showing statistical differences among the lineages. In this work, the establishment and multiplication of embryogenic calli of E. precatoria are described for the first time, and they emerge as viable alternatives for the vegetative propagation of the species.</description><subject>Analysis</subject><subject>Biochemical analysis</subject><subject>Charcoal</subject><subject>Cultivation</subject><subject>Embryos</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Euterpe precatoria</subject><subject>Explants</subject><subject>Fruit cultivation</subject><subject>Growth kinetics</subject><subject>Kinetics</subject><subject>Leaves</subject><subject>Multiplication</subject><subject>Multivariate analysis</subject><subject>Picloram</subject><subject>Plant propagation</subject><subject>Principal components analysis</subject><subject>Propagation</subject><subject>Proteins</subject><subject>Semisolids</subject><subject>Statistical analysis</subject><subject>Sucrose</subject><subject>Suspension culture</subject><issn>1999-4907</issn><issn>1999-4907</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpNUc1q3DAQNiWFhiSHvIGgp0B2I8mSbB03y-YHNrTQ9mwm8iirYEuOJB82z9EHjpItoXOZ4eP7YWaq6pzRZV1remWZZJS1VHypjpnWeiE0bY7-m79VZyk901KyaTUXx9XfTcrwOLi0G9HnS_IwD9lNgzOQXfCXBHxPrl0wOxwLNpCVh2GfXCLBks34GPfhCb0zZOs8foB5h2Q1wmvwDjz5CcNINnPGOCGZIhbbEB2QB4h5SWbfYyS_5jShTyWOrEv6HPG0-mphSHj2r59Uf242v9d3i-2P2_v1arswnLO8aGtrwKBF00BvhYKGW6U0SNG0kttWU6UYGqEk7xF5w1DLum8ktkxyELY-qb4ffKcYXmZMuXsOcywbpq5mnCopFRWFtTywnmDAznkbcoQSDP37TYJH6wq-aplgrGm5KoKLg8DEkFJE203RjRD3HaPd-6O6z0fVb2w3htI</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>Vasconcelos, Alexandro Dias Martins</creator><creator>Ferreira, Jéssica Cristina Barbosa</creator><creator>Meira, Rennan Oliveira</creator><creator>Silva-Cardoso, Inaê Mariê de Araújo</creator><creator>Neves, Joane dos Santos</creator><creator>Souza, Anderson Marcos de</creator><creator>Souza, André Luís Xavier de</creator><creator>Scherwinski-Pereira, Jonny Everson</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><orcidid>https://orcid.org/0000-0001-6271-332X</orcidid><orcidid>https://orcid.org/0000-0002-5582-5371</orcidid></search><sort><creationdate>20241001</creationdate><title>Establishment, Multiplication, and Biochemical Analysis of Embryogenic Lines of the Amazonian Palm Euterpe precatoria Mart. under Suspension Culture</title><author>Vasconcelos, Alexandro Dias Martins ; Ferreira, Jéssica Cristina Barbosa ; Meira, Rennan Oliveira ; Silva-Cardoso, Inaê Mariê de Araújo ; Neves, Joane dos Santos ; Souza, Anderson Marcos de ; Souza, André Luís Xavier de ; Scherwinski-Pereira, Jonny Everson</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c221t-83fcacefec7adf46a72f669a547852f890661ec4652dee271e953d75e8152a4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analysis</topic><topic>Biochemical analysis</topic><topic>Charcoal</topic><topic>Cultivation</topic><topic>Embryos</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Euterpe precatoria</topic><topic>Explants</topic><topic>Fruit cultivation</topic><topic>Growth kinetics</topic><topic>Kinetics</topic><topic>Leaves</topic><topic>Multiplication</topic><topic>Multivariate analysis</topic><topic>Picloram</topic><topic>Plant propagation</topic><topic>Principal components analysis</topic><topic>Propagation</topic><topic>Proteins</topic><topic>Semisolids</topic><topic>Statistical analysis</topic><topic>Sucrose</topic><topic>Suspension culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vasconcelos, Alexandro Dias Martins</creatorcontrib><creatorcontrib>Ferreira, Jéssica Cristina Barbosa</creatorcontrib><creatorcontrib>Meira, Rennan Oliveira</creatorcontrib><creatorcontrib>Silva-Cardoso, Inaê Mariê de Araújo</creatorcontrib><creatorcontrib>Neves, Joane dos Santos</creatorcontrib><creatorcontrib>Souza, Anderson Marcos de</creatorcontrib><creatorcontrib>Souza, André Luís Xavier de</creatorcontrib><creatorcontrib>Scherwinski-Pereira, Jonny Everson</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Agricultural Science Database</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><jtitle>Forests</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vasconcelos, Alexandro Dias Martins</au><au>Ferreira, Jéssica Cristina Barbosa</au><au>Meira, Rennan Oliveira</au><au>Silva-Cardoso, Inaê Mariê de Araújo</au><au>Neves, Joane dos Santos</au><au>Souza, Anderson Marcos de</au><au>Souza, André Luís Xavier de</au><au>Scherwinski-Pereira, Jonny Everson</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment, Multiplication, and Biochemical Analysis of Embryogenic Lines of the Amazonian Palm Euterpe precatoria Mart. under Suspension Culture</atitle><jtitle>Forests</jtitle><date>2024-10-01</date><risdate>2024</risdate><volume>15</volume><issue>10</issue><spage>1804</spage><pages>1804-</pages><issn>1999-4907</issn><eissn>1999-4907</eissn><abstract>The palm Euterpe precatoria holds great social, cultural, and environmental importance. The heart of palm and the fruit are the main products used for industrialization due to their energetic properties. Thus, the aim of this study was to establish a suspension cultivation protocol for the species using different explant sources. For this, eight lineages of E. precatoria embryogenic calluses were tested, with five in liquid medium Murashige and Skoog (MS) with 5 μM Picloram and three for comparison in semisolid medium MS with 20 μM Picloram and 5 μM 2iP. The growth curve was obtained by weighing the calli from 60 to 180 days of cultivation. The Gompertz model was applied, and growth kinetics were evaluated. At 100 days, the contents of total soluble sugars (TSSs) and total soluble proteins (TSPs) were determined. Principal components (PCA) were measured. According to the analysis of the data, the cultivation of E. precatoria lineages in liquid medium was successfully carried out, and the establishment was achieved. The model can be considered adequate since the R2 values found describe more than 90% of the growth kinetics of the lineages. In the liquid system, lineages L1 (from leaf explants and multiplied in semisolid medium—SM), L2 (from leaf explants and multiplied in SM), and L6 (from zygotic embryo explants and multiplied in liquid medium—LM) showed the shortest time to double the biomass accumulation. Multivariate analysis reveals a significant increase in masses in liquid cultures, represented by lineages L6 and L2. There was statistical difference in the amount of TSSs extracted; the highest TSS levels were observed in lineages cultivated in LM. The protein content found was very low, showing statistical differences among the lineages. In this work, the establishment and multiplication of embryogenic calli of E. precatoria are described for the first time, and they emerge as viable alternatives for the vegetative propagation of the species.</abstract><cop>Basel</cop><pub>MDPI AG</pub><doi>10.3390/f15101804</doi><orcidid>https://orcid.org/0000-0001-6271-332X</orcidid><orcidid>https://orcid.org/0000-0002-5582-5371</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Analysis Biochemical analysis Charcoal Cultivation Embryos Ethylenediaminetetraacetic acid Euterpe precatoria Explants Fruit cultivation Growth kinetics Kinetics Leaves Multiplication Multivariate analysis Picloram Plant propagation Principal components analysis Propagation Proteins Semisolids Statistical analysis Sucrose Suspension culture |
title | Establishment, Multiplication, and Biochemical Analysis of Embryogenic Lines of the Amazonian Palm Euterpe precatoria Mart. under Suspension Culture |
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