Development of a highly sensitive CNT-metal graphene hybrid nano-IDA electrochemical biosensor for the diagnosis of Alzheimer's disease
The blood-based detection of Alzheimer's disease (AD) is becoming challenging since the blood-brain barrier (BBB) restricts the direct circulation of AD molecules in the blood, thereby precluding the detection of AD at an early-stage. Herein, we report the development of a novel CNT-metal-porou...
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Veröffentlicht in: | Biomaterials science 2024-10, Vol.12 (2), p.523-5214 |
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Sprache: | eng |
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Zusammenfassung: | The blood-based detection of Alzheimer's disease (AD) is becoming challenging since the blood-brain barrier (BBB) restricts the direct circulation of AD molecules in the blood, thereby precluding the detection of AD at an early-stage. Herein, we report the development of a novel CNT-metal-porous graphene hybrid (CNT-MGH) nano-interdigitated array (n-IDA) electrochemical 8-well biosensor for the successful early-stage diagnosis of AD from blood. Laser-induced graphene (LIG) technology has been used to fabricate the proposed CNT-MGH n-IDA 8-well sensor. Firstly, the electrochemical characterization (
i.e.
, electrode gap, material composition,
etc.
) of the proposed sensor was demonstrated by measuring
p
-aminophenol (PAP) with a limit of detection (LOD) of 0.1 picomole. Subsequently, the CNT-MGH n-IDA 8-well sensor was then used to diagnose AD
via
novel blood biomarkers p-Tau 217 and p-Tau 181 using an electrochemical enzyme-linked immunosorbent assay (e-ELISA) enzyme by-product PAP. During e-ELISA, the alkaline phosphatase enzyme (IgG-AP) tagged to the detection antibody produced an electroactive ELISA by-product PAP by reacting with the enzyme-substrate 4-aminophenyl phosphate (PAPP). Finally, the CNT-MGH n-IDA 8-well sensor was then used to measure the current generated by the redox reaction
via
the e-ELISA by-product PAP. While quantified, the proposed CNT-MGH n-IDA 8-well sensor successfully detected p-Tau 217 and p-Tau 181 proteins in blood with LODs of 0.16 pg ml
−1
and 0.08 pg ml
−1
, respectively.
In this work, a novel CNT-metal-porous graphene hybrid nano-interdigitated array electrochemical 8-well biosensor has been developed for the successful early-stage diagnosis of Alzheimer's disease from blood using e-ELISA enzyme by-product PAP. |
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ISSN: | 2047-4830 2047-4849 2047-4849 |
DOI: | 10.1039/d4bm00654b |