Influence of maltose concentration on Garut sheep (Ovis aries L.) oocyte nuclear visualization after maturation

Research on the influence of maltose administration on the oocyte nuclear visualization of Garut sheep (Ovis aries L.) after maturation has been carried out. The aim of this study was to evaluate the ability of maltose and determine the optimum concentration of maltose that induces post-maturation of swollen oocyte nuclear. The study was compiled based on completely randomized design consisting of one trial, four treatment groups, and six replications. In the first experiment, to ripen the oocytes in the Metaphase II stage, observations were made with the addition of various concentrations of maltose (0 %, 1 %, 3 %, and 5 %). The parameter used for oocyte maturation was Metaphase II which was characterized by the formation of Polar Body I. The nuclear viability of swollen oocytes was observed using Hoechst & PI dyes. The results showed that statistically there were differences between concentrations based on the Shapiro-Wilk normality test, the Levene homogeneity test, and ANOVA variance test (P≥0.05). Treatment using 3 % maltose concentration showed the highest percentage of post-maturation swollen oocyte nuclear (66.14 %). Therefore, the 3 % maltose concentration is the optimum concentration to cause swollen post-maturation of oocyte nuclear.