1546-P: Autonomic Intrapancreatic Neurons Alterations in Type 1 Diabetes

Introduction: Little is known about parasympathetic intrapancreatic neurons despite their important role in regulation of endocrine and exocrine functions. 3D imaging studies show innervation of these ganglia and islets in organ donors with type 1 diabetes (T1D). This study analyzed intrapancreatic...

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Veröffentlicht in:Diabetes (New York, N.Y.) N.Y.), 2024-06, Vol.73, p.1
Hauptverfasser: Ewing, Sam, Haselden, Sydney, Nair, Malavika, Butterworth, Elizabeth A, Campbell-Thompson, Martha
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Sprache:eng
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Zusammenfassung:Introduction: Little is known about parasympathetic intrapancreatic neurons despite their important role in regulation of endocrine and exocrine functions. 3D imaging studies show innervation of these ganglia and islets in organ donors with type 1 diabetes (T1D). This study analyzed intrapancreatic ganglia from organ donors without and with T1D. Methods: Paraffin sections from donors with no diabetes (ND, n=15) or T1D (n=9) were obtained from nPOD. Multiplex immunofluorescence was performed using antibodies to HuC/D, synapsin (SYN), and allograft inhibitory factor 1 (IBA1) to define neurons, synapses, and macrophages, respectively. Confocal images from 83 ganglia were analyzed using QuPATH software to quantify each ganglia area and immunopositive areas within ganglia. Ganglion sizes were binned into four groups. Neuron analyses included area (size) and numbers/ganglion (density). Statistical comparisons were made using non-parametric t-tests. Results: A significant reduction in HuC/D+ area/ganglia was observed in T1D donors compared to ND (p < 0.01). Neuron sizes were smallest in T1D donors in the smallest ganglia (
ISSN:0012-1797
1939-327X
DOI:10.2337/db24-1546-P