Characterization of Solanum surattense Burm. F Fruit through Chromatographic, Chemical and Analytical Methods

Background:Solanum surattenseBurm.f. is an important medicinal plant in Indian traditional medicinal systems. Fruits of the plant are used for curing asthma, fever, gonorrhoea, respiratory disease and rheumatism. The aim of the study is to investigate the physicochemical, chromatographic analysis and spectral characterization of fruits of S. surattense Burm.f. Methods: Fruit powder was subjected to loss on drying, total ash, water soluble ash, acid insoluble ash, water soluble extractive, alcohol soluble extractive and pH, High performance thin layer chromatography (HPTLC), High pressure liquid chromatography (HPLC) studies were carried out for identifying the active phyto constituents. Minerals of S. surattense in the form of ash was analysed for exploring the elements through instrumental characterization such as X-ray Fluorescence (XRF), Energy Dispersive X-Ray analysis (EDAX), X-Ray Photoelectron Spectroscopy (XPS), Powder X-Ray Diffractometer (PXRD) and Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). Results:In HPTLC photo documentation under 254 nm, 366 nm and after derivatization with vanillin sulphuric acid reagent observed six, nine and six spots respectively. HPLC chromatogram at 235 nm showed separation of eleven peaks. In the characterization techniques, carbon, oxygen, magnesium, silicon, sulphur, phosphorous, sodium, potassium, chlorine, calcium were the elements identified. Conclusion:The study pay way to quality control parameters as well as mineral value of the plant.