Bio-analytical method development and validation of dichlorvos pesticide by rp-uflc method

Organophosphates are a group of pesticides that were developed in the 1940s in Germany andsoon became a sig nificant defense against agricultural pests. The organophosphate Dichlorvos is a commonly used group of pesticide is a broad-spectrum organophosphate compound having insecticidal activity. Dichlorvos is a cholinesterase inhibitor exhibiting stomach, contact and systemic mode of action. The development of bioanalytical method is the process of developing a procedure for the detection and quantification of a compound of interest in a biological fluid. A simple, reliable, fast, accurate and economical RP-UFLC method have been developed and validated for quantitative determination of Dichlorvos. The RP-UFLC procedure is developed by using ACN and Millipore water 50:50 v/v as mobile phase, Flow rate is maintained at 1.5mL/minute. Detection of Dichlorvos was performed by using PDA detector at 200nm. By this RP-UFLC procedure retention time (RT) of Dichlorvos was identified at 2.9 min.