Oxidative Stress-Related Semen Quality and Fertility in the Male Arabian Yellowfin Sea Bream (Acanthopagrus arabicus) Fed a Selenium Nanoparticle-Supplemented Plant Protein-Rich Diet

A three-month research was conducted to evaluate the influence of supplementing a plant protein- (PP-) rich diet (60% of fish meal (FM) was replaced with a mixture of alternative PP sources) with selenium nanoparticles (Se-N) on sperm kinetics, fertilization capacity, and antioxidative responses in Arabian yellowfin sea bream (Acanthopagrus arabicus) males. In this point, graded levels of Se-N at 0, 0.5, 1, 2, and 4 mg/kg were added to the PP-rich diet. Also, a FM-based diet was used as a positive control. Two hundred and eighty-eight brooders (males: 195.3±10.8 g and females: 237.5±8.1 g; mean±standard error) were randomly distributed into eighteen 10 m3 rectangular concrete tanks (8 males: 8 females in each tank), and every three tanks were fed with one of the experimental feeds for three months. The seawater temperature and salinity were 18-22°C and 40-41‰, respectively, during the husbandry period. Selenium retention increased in the liver, testes, and semen of males with increasing dietary Se-N level (P