1960-LB: A 3D Analysis of CD8+ Leukocytic Infiltration in Human Endocrine and Exocrine Pancreas at Type 1 Diabetes Onset

1960-LB: A 3D Analysis of CD8+ Leukocytic Infiltration in Human Endocrine and Exocrine Pancreas at Type 1 Diabetes Onset

Introduction: Elevations in immune cell numbers are well documented in both the endocrine and exocrine pancreas in type 1 diabetes (T1D), with CD8+ T cells representing the predominant cell type in islet inflammation (insulitis). To expand on previous 2D characterizations, we used 3D light sheet flu...

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Veröffentlicht in:Diabetes (New York, N.Y.) N.Y.), 2024-06, Vol.73 (Supplement_1), p.1
Hauptverfasser: RIPPA, ALEXANDRA, DROTAR, DENISE M., POSGAI, AMANDA, HULME, MAIGAN A., WASSERFALL, CLIVE, KUSMARTSEVA, IRINA, CAMPBELL-THOMPSON, MARTHA, ATKINSON, MARK A.
Format: Artikel
Sprache:eng
Schlagworte:
Autoantibodies
CD8 antigen
Diabetes
Diabetes mellitus (insulin dependent)
Glucagon
Infiltration
Inflammation
Insulitis
Lymphocytes T
Pancreas
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Zusammenfassung:Introduction: Elevations in immune cell numbers are well documented in both the endocrine and exocrine pancreas in type 1 diabetes (T1D), with CD8+ T cells representing the predominant cell type in islet inflammation (insulitis). To expand on previous 2D characterizations, we used 3D light sheet fluorescent microscopy (LSFM) to quantify CD8+ cells within islets and surrounding exocrine regions. Methods: Pancreas samples from non-diabetic controls (ND, n = 4; age range 16-24 yr), non-diabetic GAD autoantibody-positive (GADA, n = 3; 18-24 yr), and recent-onset (duration
ISSN:0012-1797
1939-327X
DOI:10.2337/db24-1960-LB