Occurrence, isolation, and identification of emergent pathogens of taxon Vibrio from the Danube river in Bratislava city, Slovakia
The occurrence of Vibrio cholerae , Vibrio metschnikovii and Vibrio fluvialis , important aquatic human pathogens of genus Vibrio , were determined in the surface water of the Danube River in Bratislava. The isolates were identified by biochemical tests. Biochemical characteristics of environmental...
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Veröffentlicht in: | Biológia 2024-09, Vol.79 (9), p.2863-2872 |
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Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The occurrence of
Vibrio cholerae
,
Vibrio metschnikovii
and
Vibrio fluvialis
, important aquatic human pathogens of genus
Vibrio
, were determined in the surface water of the Danube River in Bratislava. The isolates were identified by biochemical tests. Biochemical characteristics of environmental isolates could be slightly different from reference and clinical strains which is reflected in dissimilarity rate, which was in our case not sufficient. A molecular method called amplified ribosomal DNA restriction analysis was used for confirmation of phenotypic identification. The species identity of 19 out of 22 isolates was confirmed, in the case of 3 misidentified ones, the identity of the genus did not change. The identity of all
V. cholerae
isolates was confirmed by PCR using species-specific gene called
ompW
. Also, main virulence factors of these isolates were determined. None of the isolates possessed all three virulence factors necessary for the induction of cholera. |
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ISSN: | 1336-9563 0006-3088 1336-9563 |
DOI: | 10.1007/s11756-024-01732-3 |