Isolation and screening of actinomycetes producing antimicrobial substances from Pulau Seribu

Indonesia is a maritime country with a water area of up to 75%. Microorganisms have a relatively high level of biodiversity, but their potential has yet to be thoroughly explored. The need for raw materials for medicines, especially antibiotics, continues to grow along with the population’s development and increasing antibiotic resistance. Exploration of new drugs (drug discovery) continues to be carried out to obtain lead compounds for developing new drugs. Antimicrobial compounds produced by actinomycetes have been isolated and screened. Actinomycetes isolation was taken from several marine snail samples in Pulau Seribu, Jakarta, Indonesia. The isolation was obtained from 32 actinomycetes isolates. The bioassay results on the 4 test bacteria were five isolates showed antibacterial activity. Isolates ACT134, ACT135, and ACT137 active against Bacillus subtilis and Staphylococcus aureus . Isolate ACT129 and ACT139 active against Staphylococcus aureus . Isolate ACT134 showed the isolates with the most potent inhibitory activity. Identification using 16S rRNA revealed that isolate ACT134 was Streptomyces rubiginosohelvolus , isolate ACT135 was Streptomyces bikiniensis , isolate 129 was Streptomyces albogriseolus , isolate ACT137 was Streptomyces enissocaesilis , and isolate ACT139 was Streptomyces griseorubens .