Effects of Soil Properties and Stand Factors on nirK-Denitrifying Microbial Community in Songshan, Beijing

[ Objective] This work aims to explore the traits and influence factors of soil nirK-type denitrifying microbial community in different forests in Songshan, Beijing, providing novel insights into the nitrogen cycling process and environmental change of forest soil ecosystem in the warm temperate forest soil ecosystem of North China. [Method]Three typical forest types of Pinus tabuliformis, Populus davidiana and Quercus mongolica were selected in the Songshan Nature Reserve of Beijing. Three standard sample plots(20 m×20 m) were set up in each forest type for investigation. The 0-20 cm surface mixed soil samples were collected to analyze soil properties, and extract total soil microbial DNA, PCR was used to amplify nirK gene coding the key enzyme, nitrate reductase, in the denitrification process. The second generation high-throughput sequencing was used to analyze the composition and diversity of soil denitrifying microbial communities in the different forest types, and to explore the effects of soil properti