Electrochemical sensing of lactochrome in pharmaceutical sample using L-asparagine layered carbon based sensor

The electrochemical behavior of Lactochrome (LC) was investigated using Poly (L-asparagine) modified carbon paste sensor (PAMCPS) via Differential Pulse Voltammetry (DPV) and Cyclic Voltammetry (CV). PAMCPS was fabricated from the bare carbon paste sensor (BCPS) and both the bare and the modified sensors were characterized via Scanning Electron Microscope (SEM), Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) and the surface behavior and conductivity was studied. The modified sensor portrayed greater efficiency in the detection of LC in comparison with the BCPS. PAMCPS showed a noticeable electrocatalytic action for the electrochemical behavior of LC in a phosphate buffer solution (PBS) of pH 6.5 exhibiting a maximum oxidation peak current of 7.98 µA. PAMCPS showed enhanced active surface area with an appreciable rate of electron transfer when compared to BCPS resulting in a low limit of detection (LOD) of 0.25 µM in the linear range of 0.6 µM − 1.0 µM. Simultaneous analysis of LC and dopamine (DN) was made to verify the efficiency of the proposed sensor. The impact of common interferents like metal ions, inorganic ions and biomolecules were examined and no effect on the peak current of LC was observed. The newly modified sensor was employed for the detection of LC in pharmaceutical sample with a convincing recovery rate. The modified sensor portrayed outstanding stability, repeatability along with reproducibility proving it to be a stable and a well-constructed sensor for LC analysis.