Identification and molecular mechanisms of novel antioxidant peptides in black soldier fly larvae protein: simulated gastrointestinal digestion, molecular docking and in vitro/in vivo studies

Summary This study aims to identify novel antioxidant peptides within black soldier fly larvae protein (BSFLP) using simulated gastrointestinal processes, LC–MS/MS analysis, and virtual screening. Two peptides, PFCPK and ADFW, exhibited significant antioxidant properties in both in vitro radical scavenging experiments and in vivo evaluations using zebrafish embryos induced with 2,2′‐azobis(2‐methylpropionamidine) dihydrochloride. Treatment with 25 μg mL−1 of PFCPK or ADFW significantly (P