Effects of Chloral Hydrate on the Morphogenetic Characteristics of the Neocortex and Functional Status in Elderly Male Rats
There is a need to take the characteristics and possible influences of animals’ age and the anesthesia used in experiments on their outcomes into account to avoid producing distorted results at the preclinical research stage of new pharmaceutical substances and therapeutic strategies. This paper rep...
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Veröffentlicht in: | Neuroscience and behavioral physiology 2024-03, Vol.54 (3), p.474-481 |
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Sprache: | eng |
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Zusammenfassung: | There is a need to take the characteristics and possible influences of animals’ age and the anesthesia used in experiments on their outcomes into account to avoid producing distorted results at the preclinical research stage of new pharmaceutical substances and therapeutic strategies. This paper reports the effects of chloral hydrate on the morphological features of neurons, the reactions of neocortical microglia, and the functional status of elderly male Wistar rats (age 24 months). Differences were assessed in control rats and in the group given chloral hydrate at the dose required to achieve anesthesia (400 mg/kg body weight) 48 h after its administration. After administration of chloral hydrate, surviving animals (mortality rate 37.5%) demonstrated significant increases in signs of neurological deficit in the form of motor and behavioral disorders as compared with rats of the control group. The frontoparietal region of the neocortex of surviving animals showed significantly greater levels of Iba-1 protein expression than controls; neurons and microgliocytes with marked morphological changes were noted, though the numbers of these cells were not significantly different from those in the control group. The results obtained here suggest that the unfavorable effects of chloral hydrate in the early post-anesthesia period in elderly rats, apparent as deterioration in functional status and mortality, may be mediated by activation of microglia in the frontoparietal region of the neocortex. The question of the extent to which microglial activation in specific brain structures is associated with the formation of neurological disorders requires further study. The results reported here should be taken into account in basic and preclinical studies in which elderly rats are used for research with chloral hydrate as anesthetic. |
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ISSN: | 0097-0549 1573-899X |
DOI: | 10.1007/s11055-024-01614-w |