Analysis of Enzyme and Gene Expression Related to Sugar Metabolism in Lycium barbarum under Elevated CO2 Concentration Treatment

[Objective] This study aims to explore the effect of elevated CO2 concentration on carbohydrate accumulation during the development of Lycium barbarum fruits,so as to provide a reference for adaptive management of L. barbarum to cope with climate change.[Method]L. barbarum 'Ningqi 1' was used as the experimental material. An open-top chamber simulation control system was applied to elevate the CO2 concentration(800±20 μmol·mol-1), with the ambient CO2 concentration(400±20 μmol·mol-1) served as the control. After 60 days of treatment, the root, stem, leaf, and fruit samples were collected respectively at the young fruit stage, green fruit stage, coloring fruit stage, and red fruit stage, and the content of their sugar components, sugar metabolism-related enzyme activity, and relevant gene expression were determined.[Result]1) The elevated CO2 concentration promoted the accumulation of lactose, sucrose, and total sugars during the fruit development, and at the red fruit stage, the content of lactose, fructose,