Simultaneous Quantification of 39 Pesticides and Veterinary Drug Residues in Aquaculture Products Using Ultra Performance Liquid Chromatography Tandem Mass Spectrometry with Modified QuEChERS

The aim of this work was to establish a cost-effective extraction procedure for simultaneous determination of pesticides and veterinary drugs in Chinese market preference fresh aquatic products based on MRLs in food of animal origin in China. Modified QuEChERS method coupled to UPLC-MS/MS for the simultaneous detection of 39 pesticides and veterinary drugs were developed. Six representative matrices of bulk fresh aquatic products in China’s coastal markets showed applicability with recoveries 60.3–119.0% and acceptable precision 4.3–20.8%. Experiments showed that for fresh aquatic product matrices, the addition of EDTA-Mcllvaine effectively improved the extraction efficiency, and 0.4-g C 18 /0.4 g PSA/1.2-g MgSO 4 combination with n-hexane extraction three times can remove the matrix interference effectively. Furthermore, LOD and LOQ in the range of 1.0–5.0 μg/kg and 3.0–10.0 μg/kg for 39 pesticides and veterinary drugs upon six representative matrices of bulk fresh aquatic products, respectively, that providing a practical flexible screening method for production quality control before entering the market.