Phytohormone and amino acid content in golden apple snail (Pomaceae canaliculata L.) extract which fermented at several effective microorganism 4 (EM4) concentrations

This study aims to determine the phytohormone and amino acid content of golden apple snail (GAS) extract fermented using several concentrations of EM 4 Active. GAS were taken from rice fields in Tegalgede Village, Jember. Analysis of phytohormone and amino acid content using LCMS at Bioscience laboratory, Politeknik Negeri Jember. EM4 was activated for 7 days using 5% sugar with distilled water as a solvent. The active EM4 results were taken in several concentrations: 0, 5, 10, 15, 20 and 25% and fermented in GAS extract for 14 days. This research was designed using CRD non-factorial design, consisting of 6 Active EM4 concentrations and repeated 4 times. The results of the research showed that there was the phytohormone IAA (Indole Acetic Acid) with the highest concentration of 0.838% in GAS extract fermented using 25% active EM4, while fermentation without EM4 (0%) contained only 0.641% IAA. Other analysis results showed that 12 types of amino acids were detected, namely arginine, tyrosine, alanine, cysteine, glutamic acid, methionine, glycine, leucine-isoleucine, aspartic acid, proline, threonine and valine. The analysis results showed that the 0% (control) treatment had a higher average amino acid value than fermentation using EM4.