One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity

This study introduces a one-pot isothermal amplification assay for ultrasensitive analysis of terminal deoxynucleotidyl transferase (TdT) activity. The system realizes recycled activation of CRISPR/Cas12a, enabling exceptional signal amplification. This approach maximizes the simplicity of the detec...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2024-04, Vol.6 (35), p.4683-4686
Hauptverfasser: Xu, Jianguo, Yang, Haidong, Sui, Zhuqi, Yuan, Xinyue, Jia, Lee, Guo, Longhua
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Sprache:eng
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Zusammenfassung:This study introduces a one-pot isothermal amplification assay for ultrasensitive analysis of terminal deoxynucleotidyl transferase (TdT) activity. The system realizes recycled activation of CRISPR/Cas12a, enabling exceptional signal amplification. This approach maximizes the simplicity of the detection method, offering a promising avenue for molecular disease diagnosis. Target-triggered one-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity.
ISSN:1359-7345
1364-548X
DOI:10.1039/d4cc00825a