A Novel Natural Product-Derived Compound, Vestaine A 1 , Exerts both Pro-Angiogenic and Anti-Permeability Activity via a Different Pathway from VEGF

[...]a treatment to eliminate the fragile vessels by suppressing excess angiogenesis or enhancing vessel maturation would be useful for treating these clinical conditions. Recently, anti-VEGF antibodies have been approved to treat eye diseases such as age-related macular degeneration and cancer, and show steady efficacy [1, 10, 11]. [...]medicines which accomplish abnormal vessel elimination through mechanisms different from anti-VEGF therapy would be useful to provide other options for patients showing resistance or insufficient response to the anti-VEGF therapy. Power SYBR Green PCR Master Mix and Taqman Gene Expression Master Mix were purchased from Applied Biosystems. After being stirred for 15 min at that temperature, N-acetyl-L-cysteine (1.6 g, 9.67 mmol) was added to the reaction mixture and stirred for 1 h. The reaction mixture was warmed up to room temperature and stirred for 1 h. The reaction was quenched by addition of 1 N HCl (30 ml) at 0°C and extracted with ethyl acetate.