Comparative proteomics analysis of whey fractions from Korean mother's milk with different gender babies in the northeast China

Infant's gender may have influences on composition of breastmilk. This study investigated differences in whey fractions from milk of mothers (n = 12, 3 biological repeats) with male or female babies using a 4D label‐free proteomics. Altogether, 1572 proteins were identified and 42 of them were differentially expressed. Majority of the different proteins are located in extracellular position and possessed domain of immunoglobulin V‐set. The screened proteins were observed to contribute greatly to functions of cellular process, binding, and cell part; and pathways of immune system and immune disease. The findings may afford beneficial knowledge for understanding differences in breastmilk composition. Differences in whey proteins of breastmilk of women delivered male or female infants were studied using a 4D label‐free proteomic technique. Differentially expressed proteins were observed to be 42. These proteins were analysed for subcellular localization, domain, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes.