E. Coli Nissle 1917 Based Carrier for DNA Delivery into Colorectal Carcinoma Cells

Objective: In recent years, bactofection—bacteria-mediated DNA transfer into mammalian cells is considered a promising approach to cancer gene therapy. Results and Discussion: In this study, we first attempted to develop a probiotic strain Escherichia coli Nissle 1917 for transferring shuttle plasmid into colorectal carcinoma cells HCT116. For this purpose, the strain was modified by inv and hly genes from Yersinia enterocolitica and Listeria monocytogenes . Conclusions: Although the efficiency of HCT116 transfection by GFP-plasmid was exceptionally low, the results demonstrated the potential use the modified probiotic strain for bactofection.