Lavender officinalis essential oil conjugated carboxymethyl cellulose: as boosters of antibacterial and bio enhancers to accelerate the repair of full-thickness infected wound

Biodegradable polymers can be utilized for coating plant derivations and help to preserve their medicinal properties, such as carboxymethyl cellulose (CMC). This study was conducted to investigate the wound healing activity of nanoemulsions (NEs) of Lavender officinalis essential oil (LOEO)/CMC in an infected mice model. Physicochemical properties of the NEs of LOEO and LOEO-NES/CMC-ONT were investigated. The NEs were blended with base formulations and administrated on infected wounds. Wound contraction, histopathological parameters, the expressions of CD31 and VEGF (Vascular endothelial growth factor), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), smooth muscle alpha-actin (αSMA), transforming growth factor-β (TGF-β) and fibroblast growth factor-2 (FGF-2) were investigated. The results confirmed the successful synthesis and safety of the NEs. In addition, CMC helped to slow release and in a longer time of LOEO. Antibacterial activity of NEs and their ointment was confirmed. The results also showed that the administration of ointments prepared from LOEO-NEs/CMC and LOEO-NEs could increase the expression of CD31, VEGF, αSMA, TGF-β, FGF-2, and decrease TNF-α and IL-6 compared with mice treated with base formulations ( p = 0.0001). Ointments prepared from LOEO-NEs/CMC and LOEO-NEs accelerate wound healing via promoting the proliferative phase and especially via factors involved in angiogenesis.