Functionalized single‐walled carbon nanotubes electrochemical aptasensor for human epidermal growth factor receptor 2 (HER2) breast cancer detection

In this study, we present a novel functionalized single‐walled carbon nanotubes (SWCNTs) electrochemical aptasensor designed for the detection of HER2 breast cancer. The well‐organized SWCNTs were attained through a concentration‐based and single‐step ultrasonication‐directed assembly methodology, resulting in a well‐dispersed state and a thin, even coating on the transducer substrate. Well‐organized SWCNTs were systematically analyzed through X‐ray diffraction spectroscopy (XRD), Fourier transforms infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy and elemental mapping (TEM‐mapping) and X‐ray photoelectron spectroscopy (XPS) confirmed the superior quality and organization of the SWCNTs. Furthermore, the electrochemical sensor exhibited outstanding performance, showcasing the potential of the SWCNTs as an ideal material for such applications, including their anticancer activity against human breast cancer cells. The single‐step ultrasonication‐directed assembly approach not only enhances the electrochemical properties of SWCNT but holds promise as a potentially as versatile substrate for immobilizing enzymes, antibodies, and DNA. This versatility opens up opportunities for the creation of highly functional biosensors with broad medical applications. Overall, our findings highlight the scientific significance of SWCNTs in advancing electrochemical sensing technologies and their potential in combating breast cancer.