Liquid Static Culture Method Using Highly Oxygen-Permeable Film

We propose a culture method using bags composed of polymethylpentene (TPX) film, which has high oxygen permeability. A liquid medium in a TPX bag was inoculated with a recombinant Bacillus choshinensis that secretes a recombinant peroxidase. The culture was maintained under non-shaking conditions. The B. choshinensis culture in the TPX bag exhibited higher enzyme activity than a corresponding B. choshinensis culture shaken in a conical tube. Thus, the TPX film bag can be used for liquid culturing under aerobic conditions.