Peculiarities of Cytochrome c Enzymatic Activity with Cardiolipin

The points of enzymatic activity, quantum yields, structure, functions of luminol chemiluminescence activated by physical activators natural dyes isoquinolysine coumarins C-334 and C-525 under the action of cytochrome c complex with cardiolipin in aqueous medium and in nonpolar environment were considered. It is shown: (1) the enzymatic activity points and quantum yields are significantly higher in the presence of the physical activator coumarin C-525 than in the case of its own unactivated luminescence or in the case of the physical activator C-334; (2) the enzymatic activity depends not only on the concentration of cytochrome c but also on the percentage ratio between the native form of cytochrome c with cardiolipin and the partially denatured one.