Bacterial Strains from the Bioresource Collection of the Federal State Budgetary Scientific Institution of the Federal Scientific Center for Biological Plant Protection, which Have Growth-Stimulating Activity against Winter Wheat Plants

The studies were carried out to search for bacterial strains with growth-stimulating activity. Eighty strains were evaluated during the experiments. The Batko winter wheat variety was used as a test plant. The work was carried out in a greenhouse. Bacterial cultures were grown on potato-glucose agar at +28°C. The bacterial mass was washed off from Petri dishes, diluted with water up to 50 mL (titer of suspensions was 10 7 –10 9 CFU/mL). The seeds were kept in an aqueous suspension for 2 hours at +24–28°C. Fitosporin-M, W ( Bacillus subtilis 26D) and Extrasol, W ( B. subtilis Ch-13) were used as a biological standard; the control was treated with distilled water. Thirty seeds were sown in cups with sand with a volume of 0.45 L. The experiment was performed in three replicates. Counting was performed on the 14th day of incubation. The use of strains B. subtilis BZR 862, B. subtilis BZR 523-1, B. subtilis BZR 336s, Pseudomonas fulva BZR 523-2, BZR 47, BZR 845, BZR 921, BZR 747, BZR 538, BZR 462 provided an increase in shoot length, relative to the control, by 10.8–29.7%, shoot weight increased by 13.7–34.9%, root length increased by 16.8–18.2%, root weight increased by 41.9%, compared with standards, which increased by 42.0, 78.3, 33.4, and 34.2%, respectively. In experiments on growth stimulation of the dynamics, over 10 days of vegetation, strains that exhibited maximum activity, B. subtilis BZR 517, Myroides odoratimimus BZR 474, B. pumilus BZR 673, BZR 519, had the greatest positive effect on plant growth. Compared to the control, there was an increase in the length of the shoot by 13.7–21.0%, the length of the root increased by 16.4–40.9%, the mass of the shoot increased by 43.5–98.0%, and the mass of the root increased by 25.3–54.0%.