Determination of LD50 value of gamma ray iradiation for cayenne pepper (Capsicum frutescens L.)

The development of superior cayenne pepper varieties needs to be carried out to meet the increasing needs of the community. One way that can be done in the development of these superior varieties is through mutation induction techniques with gamma-ray irradiation. This research aims to study the growth response and determine the LD50 of cayenne pepper sprouts of Rabani Agrihorti and Prima Agrihorti varieties due to Gamma-ray irradiation. The experimental design used was a completely randomized design, 4 replications consisting of two factors, namely varieties of cayenne pepper (Rabani Agrihorti and Prima Agrihorti) and gamma-ray radiation dose (D0=0 Gy (control), D1=20 Gy, D2=40 Gy, D3=60 Gy, D4=80 Gy). The results indicated that the increased dose of gamma-ray irradiation can reduce the growth of invitro shoots of Rabani Agrihorti and Prima Agrihorti varieties. The LD50 value of the in vitro shoots of the Prima Agrihorti variety (17.51 Gy) was higher than the Rabani Agrihorti variety with the LD50 value at 15.45 Gy the LD50 value of gamma-ray irradiation can be used to create mutants with a high level of diversity, thereby increasing the likelihood of obtaining the desired trait.