Biomarkers of a Football Match-play - Internal load analysis using Technical Soccer Specific Aerobic Field Test (TSAFT90)

[LANGUAGE= "English"] INTRODUCTION: The study measured the magnitude of physiological, immune, endocrine, and muscle damage markers of exercise-induced fatigue using the Technical Soccer Specific Aerobic Field Test (TSAFT90). We examined the effect of fatigue on performance and recovery at 24 hours post-exercise using biochemical indices.METHODS: Professional football players (n=30) with a mean age of 19.20 years participated in the study during their preseason. To induce fatigue, participants underwent a 90-minute fatigue simulation program, TSAFT90. Venous blood samples were collected at baseline, 0-hour, and 24 hours post-fatigue. Analyzed markers included fatigue metabolites (lactate and uric acid), endocrine response marker (cortisol), muscle damage marker (creatine kinase), immunological markers (leukocytes, lymphocytes, neutrophils, and monocytes), inflammatory marker (CRP), hydration indicator (serum osmolality), and recovery marker (magnesium). Ball velocity and a 7-point Likert scale for muscle soreness were recorded to assess performance and perception of fatigue, respectively.RESULTS: All biomarkers studied were significantly elevated (p