Genome Constitution and Classification Using Retrotransposon-­Based Markers in the Orphan Crop Banana

Genome Constitution and Classification Using Retrotransposon-­Based Markers in the Orphan Crop Banana

We have exploited the repetitive and dispersed nature of many long terminal repeat (LTR)-retrotransposon families for characterizing genome constitutions and classifying cultivars of the genus Musa. Insertional polymorphisms of the elements were studied using seven published and two newly designed p...

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Veröffentlicht in:Journal of plant biology = Singmul Hakhoe chi 2005-03, Vol.48 (1), p.96-105
Hauptverfasser: Teo, Chee How (Universiti Putra Malaysia, Selangor, Malaysia), Tan, Siang Hee (Sime Darby Technology Centre Sdn Bhd, Selangor, Malaysia), E-mail: sianqtsh@simedarby.com, Ho, Chai Ling (Universiti Putra Malaysia, Selangor, Malaysia), Faridah, Qamaruz Zaman (Universiti Putra Malaysia, Selangor, Malaysia), Othman, Yasmin Rofina (Universiti Malaya, Kuala Lumpur, Malaysia), Heslop-Harrison, John Seymour (University of Leicester, Leicester, United Kingdom), Kalendar, Ruslan (University of Helsinki, Helsinki, Finland), Schulman, Alan Howard (University of Helsinki, Helsinki, Finland)
Format: Artikel
Sprache:eng
Schlagworte:
Amplification
Classification
Cultivars
Fruits
Gene polymorphism
genome constitution
Genomes
Genotypes
Hybrids
Inter-Retrotransposon Amplified Polymorphism
Long terminal repeat
MUSA
Musa acuminata
Musa balbisiana
phylogenetic analysis
Phylogeny
Plant breeding
Polymorphism
retrotransposon
RNA-directed DNA polymerase
Tissue culture
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Beschreibung
Zusammenfassung:We have exploited the repetitive and dispersed nature of many long terminal repeat (LTR)-retrotransposon families for characterizing genome constitutions and classifying cultivars of the genus Musa. Insertional polymorphisms of the elements were studied using seven published and two newly designed primers facing outwards from the LTRs and reverse transcriptase (RT) domain of the retrotransposon. The primers generated specific amplification patterns showing the universal applicability of this marker type.
ISSN:1226-9239
1867-0725
DOI:10.1007/BF03030568