Isolation and characterization of biogenic calcium carbonate/phosphate from oral bacteria and their adhesion studies on YSZ-coated titanium substrate for dental implant application

Biogenic calcium carbonate/phosphate were isolated and characterized from oral bacteria (CPOB). The crystalline nature and morphology of calcium carbonate/phosphate were characterized by X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM), respectively. XRD analysis revealed the cubic phase of YSZ coating as well as biogenic calcium carbonate (rhombohedral) and calcium phosphate oxide (hexagonal) was observed from CPOB. FESEM confirmed the extracellular synthesis of calcium compounds. Bacterial adhesion result reveals that YSZ coating drastically reduce bacterial invasion than titanium substrate.