H2O2 biosensor based on horseradish peroxidase immobilized onto the zinc sulphide–graphene–chitosan modified carbon paper electrode

A simple and novel sensor of hydrogen peroxide (H 2 O 2 ) was designed successfully. Horseradish peroxidase (HRP) is effectively immobilized on the surface of chitosan (CHI)-stabilized zinc sulphide (ZnS)/graphene (G) nanocomposites modified carbon paper electrode (CPE). The interaction between HRP enzyme and H 2 O 2 was studied from modified CPE. The catalytic performance of the biosensor was examined using cyclic voltammetry (CV) and the obtained results indicated that the prepared ZnS/G/CHI/HRP/NA/CPE nanocomposites material holds excellent catalytic performance for the H 2 O 2 detection. The proposed biosensor showed good analytical performance and long-term storage stability. The repeatability of the sensor is checked up to eight cycles.