Immuno-Localization of Peroxidase in Pumpkin (Cucurbita ficifolia Bouche) Seedlings Exposed to High-Dose Gamma Ray

Peroxidases (PODs) were localized in pumpkin tissues by using an immuno-gold labeling technique in combination with transmission electron microcopy (TEM). Polyclonal antibodies raised in rabbits against horseradish peroxidase were utilized. The localization patterns of gold particles for peroxidases from tissues of both control and gamma-irradiated plants were typically present in the plasma membrane, cytosol, and cell walls. However, particle densities were remarkably increased on the cell corner middle lamellae of parenchyma cells, especially in the petioles.