Gene polymorphism and levels of pro-inflammatory cytokines in plasma and synovial fluid of patients with posttraumatic knee osteoarthritis

Posttraumatic knee osteoarthritis patients ( n = 117) and healthy individuals ( n = 94) were investigated to determine the genotype and allelic frequency distribution of polymorphic loci Т-31С , С + 3953 Т IL -1β and G-308A TNF -α. The plasma and synovial fluid levels of proinflammatory cytokines IL -1β and TNF -α were also measured in 31 patients. The study demonstrated that the plasma and synovial fluid levels of IL -1β but not TNF -α were increased in patients with posttraumatic knee osteoarthritis. Also, the linkage between TNF -α levels and polymorphic locus G-308A TNF -α was shown: the presence of allele–308А was associated with decreased TNF -α levels. It was found that polymorphic loci Т-31С, С+3953Т IL -1β, and G -308A TNF -α are not markers of predisposition to posttraumatic knee osteoarthritis in Russian population of Rostov Oblast.