Study on the antipyretic mechanism of large pushing Tianheshui for young rabbits with endotoxin-induced fever

Objective To observe the effect of large pushing Tianheshui manipulation intervention on the body temperature of young rabbits with endotoxin-induced fever and discuss its antipyretic mechanism. Methods Thirty-two young rabbits meeting the standards were selected from 40 ordinary young male New Zealand rabbits after being adapted for 7 d, and randomly divided into a normal group, a model group, a large pushing Tianheshui group, and an ibuprofen group according to the random number table method, with 8 rabbits in each group. Rabbits in the model group, the large pushing Tianheshui group, and the ibuprofen group were subjected to preparing the endotoxin-induced fever model by intravenous lipopolysaccharide from the marginal ear vein. Rabbits in the large pushing Tianheshui group received Tuina (Chinese therapeutic massage) manipulation intervention 1.5 h and 2.5 h after modeling, respectively. Rabbits in the ibuprofen group were intragastrically given ibuprofen suspension 1.5 h after modeling. The dynamic changes in body temperature were observed for the young rabbits after fever modeling. Enzyme-linked immunosorbent assay was used to determine the content changes in positive mediators of hypothalamic body temperature, including prostaglandin (PG) E 2 and cyclic adenosine monophosphate (cAMP), as well as negative mediators of hypothalamic body temperature, including arginine vasopressin (AVP) and α-melanocyte stimulating hormone (α-MSH). Results The body temperature of the young rabbits in the model group was significantly higher than that in the normal group at 0.5–4.0 h, 5.0 h, and 5.5 h after modeling ( P