High-intensity interval training with probiotic supplementation decreases gene expression of NF-κβ and CXCL2 in small intestine of rats with steatosis

Purpose The aim of this study was to investigate the effects of high-intensity interval training (HIIT) with probiotic supplementation (Lactobacillus rhamnosus GG) on gene expression of NF-κβ and CXCL2 in small intestine of rats with hepatic steatosis (induced by tetracycline). Methods Forty male Wistar rats (200 ± 20 g) were randomly divided into five groups of ( n = 8 in each group): healthy control (HC), hepatic steatosis (HS), HS + HIIT, HS + probiotic (Pro) 5. HS + HIIT + Pro. Tetracycline was gavage at a dose of 140 mg/kg, for 7 days, to induce hepatic steatosis. The HIIT performed 5 sessions per week for 5 weeks on the rodent treadmill. Probiotic groups received 10 9 CFU/ml of Lactobacillus rhamnosus GG by oral gavage for 5 weeks (daily). ALT and AST were measured to confirm of HS. NF-κβ and CXCL2 genes were measured by Real-Time PCR in small intestine (jejunum). Results The results showed that there was a significant difference in gene expression of NF-κβ and CXCL2 (jejunum) between groups ( p