Celestine blue as a new indicator in electrochemical DNA biosensors

Celestine blue（CB）was introduced as a new electroactive indicator in DNA biosensors.The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods.The effect of buffer kind and p H on the electrochemical behavior of CB was studied.The peak currents of CB were linearly r...

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Veröffentlicht in:	Science China. Chemistry 2016, Vol.59 (1), p.128-134
Hauptverfasser:	Hamidi-Asl, Ezat, Raoof, Jahan Bakhsh, Meibodi, Abdolraoof Samadi, Bazgir, Zahra Haghnavaz
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosensors Chemistry Chemistry and Materials Science Chemistry/Food Science DNA浓度 DNA生物传感器 Electrochemical analysis 天青石浓度范围电化学生物传感器电化学行为相互作用紫外-可见光谱
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Beschreibung
Zusammenfassung:	Celestine blue（CB）was introduced as a new electroactive indicator in DNA biosensors.The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods.The effect of buffer kind and p H on the electrochemical behavior of CB was studied.The peak currents of CB were linearly related to DNA concentration in the range of 5.0×10^-9 to 1.0×10^-7mol/L.The detection limit of this approach was 4.76×10^-10 mol/L.Based on spectrometry data a hypochromic effect was observed in UV-Vis spectra of CB with increasing DNA concentration.The results illustrate the possible interaction mode between CB and DNA is electrostatic binding.
ISSN:	1674-7291 1869-1870
DOI:	10.1007/s11426-015-5445-3