Silver Nanoparticles Synthesized Using Eysenhardtia polystachya and Assessment of the Inhibition of Glycation in Multiple Stages In Vitro and in the Zebrafish Model
The aim was to investigate the inhibitory activities on AGE formation by testing silver nanoparticles fabricated using a methanol extract of Eysenhardtia polystachya (EP–AgNPs) and characterized using various physicochemical techniques. The in vitro glucose-albumin assay was used, and cell viability...
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description | The aim was to investigate the inhibitory activities on AGE formation by testing silver nanoparticles fabricated using a methanol extract of Eysenhardtia polystachya (EP–AgNPs) and characterized using various physicochemical techniques. The in vitro glucose-albumin assay was used, and cell viability was carried out in RAW264.7 cells. For In vivo testing, we induced diabetes in adult zebrafish with by providing a high blood glucose concentration. EP–AgNPs showed an absorption peak at 413 nm in the UV–Vis spectrum, indicating surface plasmon resonance of the nanoparticles. TEM indicated that most of the particles were spherical, with a diameter of 10–12 nm, a polydispersity index of 0.197, and a zeta potential of − 32.25 mV, suggesting high stability of the nanoparticles. The biocompatible nature of the EP–AgNPs was demonstrated in RAW264.7 cells. EP–AgNPs markedly reduced the formation of AGEs, Amadorin-product/fructosamine, Nε-(carboxymethyl)-lysine, amyloid cross β-structure, and protein carbonyl content in BSA-glucose system and increased total thiol-group after 4 weeks in hyperglycemic zebrafish, EP–AgNPs provided a protective effect against glycation. Data suggest that the inhibitory activity of EP–AgNPs on formation of AGEs is developed through a multiple-stage mechanism of glycation. EP–AgNPs could therefore be an antiglycation agent for prevention diabetic complications. |
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The in vitro glucose-albumin assay was used, and cell viability was carried out in RAW264.7 cells. For In vivo testing, we induced diabetes in adult zebrafish with by providing a high blood glucose concentration. EP–AgNPs showed an absorption peak at 413 nm in the UV–Vis spectrum, indicating surface plasmon resonance of the nanoparticles. TEM indicated that most of the particles were spherical, with a diameter of 10–12 nm, a polydispersity index of 0.197, and a zeta potential of − 32.25 mV, suggesting high stability of the nanoparticles. The biocompatible nature of the EP–AgNPs was demonstrated in RAW264.7 cells. EP–AgNPs markedly reduced the formation of AGEs, Amadorin-product/fructosamine, Nε-(carboxymethyl)-lysine, amyloid cross β-structure, and protein carbonyl content in BSA-glucose system and increased total thiol-group after 4 weeks in hyperglycemic zebrafish, EP–AgNPs provided a protective effect against glycation. Data suggest that the inhibitory activity of EP–AgNPs on formation of AGEs is developed through a multiple-stage mechanism of glycation. EP–AgNPs could therefore be an antiglycation agent for prevention diabetic complications.</description><identifier>ISSN: 1040-7278</identifier><identifier>EISSN: 1572-8862</identifier><identifier>DOI: 10.1007/s10876-018-1448-5</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Antidiabetics ; Antioxidants ; Aqueous solutions ; Biocompatibility ; Biomedical materials ; Carbonyls ; Catalysis ; Chemistry ; Chemistry and Materials Science ; Diabetes ; Enzymes ; Glucose ; In vivo methods and tests ; Inorganic Chemistry ; Lysine ; Morphology ; Nanochemistry ; Nanoparticles ; Original Paper ; Oxidative stress ; Pathogenesis ; Physical Chemistry ; Phytochemicals ; Polydispersity ; Proteins ; Silver ; Surface plasmon resonance ; Zebrafish ; Zeta potential</subject><ispartof>Journal of cluster science, 2018-11, Vol.29 (6), p.1291-1303</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-a99e10e3a15c5bcf1ffde4448062ec82dbf6997cf8b27ee6097f04ebcd49b7ba3</citedby><cites>FETCH-LOGICAL-c316t-a99e10e3a15c5bcf1ffde4448062ec82dbf6997cf8b27ee6097f04ebcd49b7ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10876-018-1448-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918292006?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,21388,21389,21390,21391,23256,27924,27925,33530,33703,33744,34005,34314,41488,42557,43659,43787,43805,43953,44067,51319,64385,64389,72469</link.rule.ids></links><search><creatorcontrib>Gutierrez, Rosa Martha Perez</creatorcontrib><creatorcontrib>Jeronimo, Felipe Fernando Martinez</creatorcontrib><creatorcontrib>Campoy, Abraham Heriberto Garcia</creatorcontrib><creatorcontrib>Vadillo, Carlos Hoyo</creatorcontrib><title>Silver Nanoparticles Synthesized Using Eysenhardtia polystachya and Assessment of the Inhibition of Glycation in Multiple Stages In Vitro and in the Zebrafish Model</title><title>Journal of cluster science</title><addtitle>J Clust Sci</addtitle><description>The aim was to investigate the inhibitory activities on AGE formation by testing silver nanoparticles fabricated using a methanol extract of Eysenhardtia polystachya (EP–AgNPs) and characterized using various physicochemical techniques. The in vitro glucose-albumin assay was used, and cell viability was carried out in RAW264.7 cells. For In vivo testing, we induced diabetes in adult zebrafish with by providing a high blood glucose concentration. EP–AgNPs showed an absorption peak at 413 nm in the UV–Vis spectrum, indicating surface plasmon resonance of the nanoparticles. TEM indicated that most of the particles were spherical, with a diameter of 10–12 nm, a polydispersity index of 0.197, and a zeta potential of − 32.25 mV, suggesting high stability of the nanoparticles. The biocompatible nature of the EP–AgNPs was demonstrated in RAW264.7 cells. EP–AgNPs markedly reduced the formation of AGEs, Amadorin-product/fructosamine, Nε-(carboxymethyl)-lysine, amyloid cross β-structure, and protein carbonyl content in BSA-glucose system and increased total thiol-group after 4 weeks in hyperglycemic zebrafish, EP–AgNPs provided a protective effect against glycation. Data suggest that the inhibitory activity of EP–AgNPs on formation of AGEs is developed through a multiple-stage mechanism of glycation. EP–AgNPs could therefore be an antiglycation agent for prevention diabetic complications.</description><subject>Antidiabetics</subject><subject>Antioxidants</subject><subject>Aqueous solutions</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Carbonyls</subject><subject>Catalysis</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Diabetes</subject><subject>Enzymes</subject><subject>Glucose</subject><subject>In vivo methods and tests</subject><subject>Inorganic Chemistry</subject><subject>Lysine</subject><subject>Morphology</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Original Paper</subject><subject>Oxidative stress</subject><subject>Pathogenesis</subject><subject>Physical Chemistry</subject><subject>Phytochemicals</subject><subject>Polydispersity</subject><subject>Proteins</subject><subject>Silver</subject><subject>Surface plasmon resonance</subject><subject>Zebrafish</subject><subject>Zeta potential</subject><issn>1040-7278</issn><issn>1572-8862</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc1u3CAUha2okZImfYDukLp2C_gHWEZRmo6UpItpuugGYXwZEzngcplK7vP0QctkKnWVFXA53znSPVX1ntGPjFLxCRmVoq8pkzVrW1l3J9U56wSvpez5m3KnLa0FF_Kseov4RClVsmnOqz9bP_-CRB5MiItJ2dsZkGzXkCdA_xtG8og-7MjNihAmk8bsDVnivGI2dloNMWEkV4iA-Awhk-hIIckmTH7w2cdwmNzOqzUvDx_I_X7OfpmBbLPZlaxNIN99TvHFqfwf8B8wJOM8TuQ-jjBfVqfOzAjv_p0X1ePnm2_XX-q7r7eb66u72jasz7VRChiFxrDOdoN1zLkR2rIN2nOwko-D65US1smBC4CeKuFoC4MdWzWIwTQX1Yej75Lizz1g1k9xn0KJ1FwxyRWntC8qdlTZFBETOL0k_2zSqhnVhzL0sQxdytCHMnRXGH5ksGjDDtJ_59ehv12RkOE</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Gutierrez, Rosa Martha Perez</creator><creator>Jeronimo, Felipe Fernando Martinez</creator><creator>Campoy, Abraham Heriberto Garcia</creator><creator>Vadillo, Carlos Hoyo</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>88I</scope><scope>8FE</scope><scope>8FG</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>M2P</scope><scope>P5Z</scope><scope>P62</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20181101</creationdate><title>Silver Nanoparticles Synthesized Using Eysenhardtia polystachya and Assessment of the Inhibition of Glycation in Multiple Stages In Vitro and in the Zebrafish Model</title><author>Gutierrez, Rosa Martha Perez ; Jeronimo, Felipe Fernando Martinez ; Campoy, Abraham Heriberto Garcia ; Vadillo, Carlos Hoyo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-a99e10e3a15c5bcf1ffde4448062ec82dbf6997cf8b27ee6097f04ebcd49b7ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antidiabetics</topic><topic>Antioxidants</topic><topic>Aqueous solutions</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Carbonyls</topic><topic>Catalysis</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Diabetes</topic><topic>Enzymes</topic><topic>Glucose</topic><topic>In vivo methods and tests</topic><topic>Inorganic Chemistry</topic><topic>Lysine</topic><topic>Morphology</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Original Paper</topic><topic>Oxidative stress</topic><topic>Pathogenesis</topic><topic>Physical Chemistry</topic><topic>Phytochemicals</topic><topic>Polydispersity</topic><topic>Proteins</topic><topic>Silver</topic><topic>Surface plasmon resonance</topic><topic>Zebrafish</topic><topic>Zeta potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gutierrez, Rosa Martha Perez</creatorcontrib><creatorcontrib>Jeronimo, Felipe Fernando Martinez</creatorcontrib><creatorcontrib>Campoy, Abraham Heriberto Garcia</creatorcontrib><creatorcontrib>Vadillo, Carlos Hoyo</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Journal of cluster science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gutierrez, Rosa Martha Perez</au><au>Jeronimo, Felipe Fernando Martinez</au><au>Campoy, Abraham Heriberto Garcia</au><au>Vadillo, Carlos Hoyo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silver Nanoparticles Synthesized Using Eysenhardtia polystachya and Assessment of the Inhibition of Glycation in Multiple Stages In Vitro and in the Zebrafish Model</atitle><jtitle>Journal of cluster science</jtitle><stitle>J Clust Sci</stitle><date>2018-11-01</date><risdate>2018</risdate><volume>29</volume><issue>6</issue><spage>1291</spage><epage>1303</epage><pages>1291-1303</pages><issn>1040-7278</issn><eissn>1572-8862</eissn><abstract>The aim was to investigate the inhibitory activities on AGE formation by testing silver nanoparticles fabricated using a methanol extract of Eysenhardtia polystachya (EP–AgNPs) and characterized using various physicochemical techniques. The in vitro glucose-albumin assay was used, and cell viability was carried out in RAW264.7 cells. For In vivo testing, we induced diabetes in adult zebrafish with by providing a high blood glucose concentration. EP–AgNPs showed an absorption peak at 413 nm in the UV–Vis spectrum, indicating surface plasmon resonance of the nanoparticles. TEM indicated that most of the particles were spherical, with a diameter of 10–12 nm, a polydispersity index of 0.197, and a zeta potential of − 32.25 mV, suggesting high stability of the nanoparticles. The biocompatible nature of the EP–AgNPs was demonstrated in RAW264.7 cells. EP–AgNPs markedly reduced the formation of AGEs, Amadorin-product/fructosamine, Nε-(carboxymethyl)-lysine, amyloid cross β-structure, and protein carbonyl content in BSA-glucose system and increased total thiol-group after 4 weeks in hyperglycemic zebrafish, EP–AgNPs provided a protective effect against glycation. Data suggest that the inhibitory activity of EP–AgNPs on formation of AGEs is developed through a multiple-stage mechanism of glycation. EP–AgNPs could therefore be an antiglycation agent for prevention diabetic complications.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s10876-018-1448-5</doi><tpages>13</tpages></addata></record> |
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subjects | Antidiabetics Antioxidants Aqueous solutions Biocompatibility Biomedical materials Carbonyls Catalysis Chemistry Chemistry and Materials Science Diabetes Enzymes Glucose In vivo methods and tests Inorganic Chemistry Lysine Morphology Nanochemistry Nanoparticles Original Paper Oxidative stress Pathogenesis Physical Chemistry Phytochemicals Polydispersity Proteins Silver Surface plasmon resonance Zebrafish Zeta potential |
title | Silver Nanoparticles Synthesized Using Eysenhardtia polystachya and Assessment of the Inhibition of Glycation in Multiple Stages In Vitro and in the Zebrafish Model |
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