Effects of biochar produced from tropical rice straw, corncob, and bamboo tree at different processing temperatures on in vitro rumen fermentation and methane production

This study aimed to evaluate the effects of biochar produced from tropical biomass resources (rice straw, corncob, and bamboo) at different processing temperatures (300, 500, and 700 °C) on in vitro rumen fermentation and methane production. Treatments were arranged as a 3 × 3 factorial design with three biomass resources and three biochar processing temperatures. Added biochar occupied 3% of the substrate (DM basic). Two hundred fifty milligrams of the air-dried substrate was incubated in 120 ml bottles, which contained 25 ml of mixed rumen fluid and buffer mineral solution. Total gas and methane production, in vitro digestibility of DM and OM, and in vitro rumen fermentation characteristics were determined at three time points of 4, 24, and 48 h of the incubation. The results showed that biomass resources and processing temperatures affected gas production at 4, 24, and 48 h after incubation ( P