Mechanism detoxification of chrom (VI) to chrom (III) by chromate reductase in Pseudomonas putida from of tannery waste through molecular docking studies

Chromate reductase producing bacteria had the potential to detoxify toxic hexavalent chromium into Cr(III) which was less toxic and insoluble in water. This mechanism was one that was carried out by microbes such as Pseudomonas putida. This bacterium survived in a liquid medium containing K2CrO4, during an incubation period of 72 hours. Pseudomonas putida isolate was able to reduce Cr (VI) by 64.31%. Bacteria had a resistance gene that was encoded in the plasmid with the gene name ChrR and the gene translates to protein chromate reductase (CHRR) or NAD(P)H dehydrogenase. Chromate reductase was able to oxidatively reduce chromium (VI) atoms/compounds to chromium (III). Chromate reductase in catalyzing chromium (VI) requires cofactors, one of which was FMN (Flavin Mono Nucleotide) or Riboflavin 5 Phosphate. This had been recorded in the UniProtKB database, through the secondary date base SWISS MODEL 3D structure of chromate reductase, it had been seen that the FMN compound had been bound to protein chromate reductase. By using molecular docking the FMN and chromate reductase bonds were seen with their affinity energies.