Development & validation of HPLC method for Dissolution of Remogliflozin Etabonate in Pharmaceutical dosage form

A simple, sensitive, precise, accurate and specific Chromatography High Performance Liquid Chrometography Dissolution method has been developed and validated for estimation of Remogliflozin Etabonate in Pharmaceutical dosage form. High Performance Liquid Chrometography method was carried out by Isocratic mode technique on a Reversed-Phase Inersil C18 (150 mm × 4.6 mm, 5 µm i.d) column. Detection wavelength was at 229 nm and mobile phase Acetonitrile: Water (70:30 % v/v). The flow rate was used 1.0 ml/min. The average retention times for Remogliflozin Etabonate were 4.7 min, respectively. The calibration curves were linear (r2 > 0.999) over the concentration rage 10-80 µg/ml for Remogliflozin Etabonate and 5-40 µg/ml. The r2 values was found to be 0.9997 Remogliflozin Etabonate, respectively. LOD was found to be 0.010 µg/ml for Remogliflozin Etabonate, respectively and LOQ was found to be 0.31 µg/ml. Suitability of Dissolution method for quantitative determination of compounds was confirmed by validation in accordance with the requirements of ICH guidelines