Design and Development of Rifampicin Nano-Bio-composite for the application of Anti-Microbial activity

In order to increase the antimicrobial activity of silver nanoparticles (AgNPs) against both Gram?positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, rifampicin antibiotic was loaded onto the AgNPs. In order to create AgNPs with a consistent prism shape, sodium borohydride (NaBH4), lactose, and sodium citrate were used in the preparation process. Finally, rifampicin was added to the produced AgNPs to functionalize them and create an AgNPs-rifampicin composite. The produced nanocomposites were characterised using a variety of analytical methods, including transmission electron microscopy (TEM) and ultraviolet-visible (UV-Vis) absorption spectra. According to the preparation procedures, AgNPs' average particle size and distribution varied slightly as seen in TEM pictures. AgNPs-rifampicin composites' antimicrobial activity was examined using the disc diffusion method. The prism AgNPs-rifampicin showed a considerable enhancement in antimicrobial activity against both kinds of bacteria, according to the results. Because of the vertexes and edges of nano prisms, which facilitate Ag ions' entry into the cell wall. Due to the different compositions of Gram-positive and Gram-negative bacteria's cell walls, AgNPs-rifampicin composites showed greater antibacterial efficacy against the former than the latter.