Detection and Suppression of Survivin (BIRC5) in Oral Squamous Cell Carcinoma Cell Line: An Invitro Study

Detection and Suppression of Survivin (BIRC5) in Oral Squamous Cell Carcinoma Cell Line: An Invitro Study

(1) Background: In the current study, we aimed to assess the effect of suppression of survivin (BIRC5) on the viability of oral squamous cell carcinoma cell line and to explore the changes in the expression of both p53 and bcl2 genes when surviving was blocked; (2) Methods: The oral squamous cell ca...

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Veröffentlicht in:NeuroQuantology 2022-01, Vol.20 (5), p.5262
Hauptverfasser: Abd El Rahman M Sharfeldeen, Abulwafa, Zeinab, Mohamed Abdelrahman Mohamed Ahmed Hussein Gaber Magdy Alabasiry Alazzazi Khaled Mohamed Elrahawy Abdelrahman Mohamed Galal Abdelhameed
Format: Artikel
Sprache:eng
Schlagworte:
Apoptosis
Biology
Cancer
Cell cycle
Cell division
Cell growth
Dentistry
Experiments
Genes
Medicine
Oral cancer
Pathology
Penicillin
Proteins
Squamous cell carcinoma
Survival
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Zusammenfassung:(1) Background: In the current study, we aimed to assess the effect of suppression of survivin (BIRC5) on the viability of oral squamous cell carcinoma cell line and to explore the changes in the expression of both p53 and bcl2 genes when surviving was blocked; (2) Methods: The oral squamous cell carcinoma cell line (HNO97) cells were transfected with si- BIRC5 then the cell viability was evaluated using the MTT assay. In addition, we investigated the effect of silencing BIRC5 as an apoptotic inhibitor protein on two apoptosis-regulating genes TP53 and BCL2. (3) Results: There was a marked decrease in cell viability when cell line was transfected with si- BIRC5, in addition to that, unlike Bcl2 which showed a marked decrease, the expression of p53 was increased
ISSN:1303-5150
DOI:10.48047/nq.2022.20.5.NQ22807