Microbial 1,2-Dehydrogenation of 6 α-Methylhydrocortisone 11-Trifluoroacetate
Transformation of 11-trifluoroacetate 6α-methylhydrocortisone (11-TFA MHC) by cells of the actinobacteria Arthrobacter (Nocardioides) was carried out in the presence of α-cyclodextrin ( α-CD ). The composition and dynamics of the accumulation of the transformation products in the culture medium at v...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2023-12, Vol.59 (6), p.919-926 |
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| container_title | Applied biochemistry and microbiology |
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| creator | Savinova, T. S. Arinbasarova, A. Yu Kazantsev, A. V. Savinova, O. S. Lukashev, N. V. |
| description | Transformation of 11-trifluoroacetate 6α-methylhydrocortisone (11-TFA MHC) by cells of the actinobacteria
Arthrobacter (Nocardioides)
was carried out in the presence of α-cyclodextrin (
α-CD
). The composition and dynamics of the accumulation of the transformation products in the culture medium at various pHs and the ratio of the α-CD/substrate were studied. It was shown that the addition of α-CD to the transformation medium at pH 7 promotes an increase in the rate of 1,2-dehydrogenation with the formation of 6 α-methylprednisolone 11-trifluoroacetate (11-TFA MPL). At pH 7, the primary process is the hydrolysis of the 11β-trifluoroacetyloxy group. In this case, the participation of α-CD in these processes as an acceptor of the trifluoroacetyl ion is not excluded. |
| doi_str_mv | 10.1134/S0003683823060133 |
| format | Article |
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Arthrobacter (Nocardioides)
was carried out in the presence of α-cyclodextrin (
α-CD
). The composition and dynamics of the accumulation of the transformation products in the culture medium at various pHs and the ratio of the α-CD/substrate were studied. It was shown that the addition of α-CD to the transformation medium at pH 7 promotes an increase in the rate of 1,2-dehydrogenation with the formation of 6 α-methylprednisolone 11-trifluoroacetate (11-TFA MPL). At pH 7, the primary process is the hydrolysis of the 11β-trifluoroacetyloxy group. In this case, the participation of α-CD in these processes as an acceptor of the trifluoroacetyl ion is not excluded.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683823060133</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Arthrobacter ; Biochemistry ; Biomedical and Life Sciences ; Cell culture ; culture media ; Cyclodextrin ; Cyclodextrins ; Dehydrogenation ; hydrolysis ; Life Sciences ; Medical Microbiology ; Methylprednisolone ; Microbiology ; Microorganisms ; Nocardioides ; Substrates ; Transformations ; Trifluoroacetates ; Trifluoroacetyl</subject><ispartof>Applied biochemistry and microbiology, 2023-12, Vol.59 (6), p.919-926</ispartof><rights>Pleiades Publishing, Inc. 2023. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2023, Vol. 59, No. 6, pp. 919–926. © Pleiades Publishing, Inc., 2023. Russian Text © The Author(s), 2023, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2023, Vol. 59, No. 6, pp. 622–630.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c301t-f4e18c58e5626b6647a4c4df1ef5e3b7643a077e784ea88554a4d1ac7dfa83593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683823060133$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683823060133$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Savinova, T. S.</creatorcontrib><creatorcontrib>Arinbasarova, A. Yu</creatorcontrib><creatorcontrib>Kazantsev, A. V.</creatorcontrib><creatorcontrib>Savinova, O. S.</creatorcontrib><creatorcontrib>Lukashev, N. V.</creatorcontrib><title>Microbial 1,2-Dehydrogenation of 6 α-Methylhydrocortisone 11-Trifluoroacetate</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>Transformation of 11-trifluoroacetate 6α-methylhydrocortisone (11-TFA MHC) by cells of the actinobacteria
Arthrobacter (Nocardioides)
was carried out in the presence of α-cyclodextrin (
α-CD
). The composition and dynamics of the accumulation of the transformation products in the culture medium at various pHs and the ratio of the α-CD/substrate were studied. It was shown that the addition of α-CD to the transformation medium at pH 7 promotes an increase in the rate of 1,2-dehydrogenation with the formation of 6 α-methylprednisolone 11-trifluoroacetate (11-TFA MPL). At pH 7, the primary process is the hydrolysis of the 11β-trifluoroacetyloxy group. In this case, the participation of α-CD in these processes as an acceptor of the trifluoroacetyl ion is not excluded.</description><subject>Arthrobacter</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell culture</subject><subject>culture media</subject><subject>Cyclodextrin</subject><subject>Cyclodextrins</subject><subject>Dehydrogenation</subject><subject>hydrolysis</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Methylprednisolone</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Nocardioides</subject><subject>Substrates</subject><subject>Transformations</subject><subject>Trifluoroacetates</subject><subject>Trifluoroacetyl</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kEtOwzAURS0EEqWwAGaRmDDA4Bd_4gxR-UotDCjjyHWf21RpXOxk0GWxEdZEQpGQQIysp3vulXUIOQV2CcDF1QtjjCvNdcqZYsD5HhmAYppylop9Muhj2ueH5CjGVXfmSucD8jQpbfCz0lQJXKT0BpfbefALrE1T-jrxLlHJxzudYLPcVl-Z9aEpo68xAaDTULqq9cEbi41p8JgcOFNFPPl-h-T17nY6eqDj5_vH0fWYWs6goU4gaCs1SpWqmVIiM8KKuQN0EvksU4IblmWYaYFGaymFEXMwNps7o7nM-ZCc73Y3wb-1GJtiXUaLVWVq9G0sOEguhRagOvTsF7rybai73xVp3lmBnPF-EHZUZyPGgK7YhHJtwrYAVvSGiz-Gu06668SOrRcYfpb_L30CHK58HQ</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Savinova, T. S.</creator><creator>Arinbasarova, A. Yu</creator><creator>Kazantsev, A. V.</creator><creator>Savinova, O. S.</creator><creator>Lukashev, N. V.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20231201</creationdate><title>Microbial 1,2-Dehydrogenation of 6 α-Methylhydrocortisone 11-Trifluoroacetate</title><author>Savinova, T. S. ; Arinbasarova, A. Yu ; Kazantsev, A. V. ; Savinova, O. S. ; Lukashev, N. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-f4e18c58e5626b6647a4c4df1ef5e3b7643a077e784ea88554a4d1ac7dfa83593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Arthrobacter</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell culture</topic><topic>culture media</topic><topic>Cyclodextrin</topic><topic>Cyclodextrins</topic><topic>Dehydrogenation</topic><topic>hydrolysis</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Methylprednisolone</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Nocardioides</topic><topic>Substrates</topic><topic>Transformations</topic><topic>Trifluoroacetates</topic><topic>Trifluoroacetyl</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Savinova, T. S.</creatorcontrib><creatorcontrib>Arinbasarova, A. Yu</creatorcontrib><creatorcontrib>Kazantsev, A. V.</creatorcontrib><creatorcontrib>Savinova, O. S.</creatorcontrib><creatorcontrib>Lukashev, N. V.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Savinova, T. S.</au><au>Arinbasarova, A. Yu</au><au>Kazantsev, A. V.</au><au>Savinova, O. S.</au><au>Lukashev, N. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbial 1,2-Dehydrogenation of 6 α-Methylhydrocortisone 11-Trifluoroacetate</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2023-12-01</date><risdate>2023</risdate><volume>59</volume><issue>6</issue><spage>919</spage><epage>926</epage><pages>919-926</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Transformation of 11-trifluoroacetate 6α-methylhydrocortisone (11-TFA MHC) by cells of the actinobacteria
Arthrobacter (Nocardioides)
was carried out in the presence of α-cyclodextrin (
α-CD
). The composition and dynamics of the accumulation of the transformation products in the culture medium at various pHs and the ratio of the α-CD/substrate were studied. It was shown that the addition of α-CD to the transformation medium at pH 7 promotes an increase in the rate of 1,2-dehydrogenation with the formation of 6 α-methylprednisolone 11-trifluoroacetate (11-TFA MPL). At pH 7, the primary process is the hydrolysis of the 11β-trifluoroacetyloxy group. In this case, the participation of α-CD in these processes as an acceptor of the trifluoroacetyl ion is not excluded.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683823060133</doi><tpages>8</tpages></addata></record> |
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| ispartof | Applied biochemistry and microbiology, 2023-12, Vol.59 (6), p.919-926 |
| issn | 0003-6838 1608-3024 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Arthrobacter Biochemistry Biomedical and Life Sciences Cell culture culture media Cyclodextrin Cyclodextrins Dehydrogenation hydrolysis Life Sciences Medical Microbiology Methylprednisolone Microbiology Microorganisms Nocardioides Substrates Transformations Trifluoroacetates Trifluoroacetyl |
| title | Microbial 1,2-Dehydrogenation of 6 α-Methylhydrocortisone 11-Trifluoroacetate |
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