Microbial 1,2-Dehydrogenation of 6 α-Methylhydrocortisone 11-Trifluoroacetate

Transformation of 11-trifluoroacetate 6α-methylhydrocortisone (11-TFA MHC) by cells of the actinobacteria Arthrobacter (Nocardioides) was carried out in the presence of α-cyclodextrin ( α-CD ). The composition and dynamics of the accumulation of the transformation products in the culture medium at various pHs and the ratio of the α-CD/substrate were studied. It was shown that the addition of α-CD to the transformation medium at pH 7 promotes an increase in the rate of 1,2-dehydrogenation with the formation of 6 α-methylprednisolone 11-trifluoroacetate (11-TFA MPL). At pH 7, the primary process is the hydrolysis of the 11β-trifluoroacetyloxy group. In this case, the participation of α-CD in these processes as an acceptor of the trifluoroacetyl ion is not excluded.