Green synthesis, characterization of gold nanoparticles using Justica wynaadensis leaf extract and its biological activities

In this experiment plant mediated method used to prepare Au nanoparticles using Justica wynaadensis leaf extract. The leaf extract solution chemically acts as the reducing agent for the synthesis of gold nanoparticles. The formation of Justica wynaadensis Au nanoparticles was immediately noticed, the colour of the reaction mixture changed from brown colour to dark purple colour and monitored by UV-visible spectroscopy at a wavelength between 500nm to 650nm for Au nano particles. Synthesized gold nanoparticles were subjected to characterization by using XRD, TEM, FTIR. TEM analysis of Au nanoparticles are in triangular shape with size 50nm. The Au nanoparticles showed significant result for antibacterial activity and tested against cell line A549, anti-diabetic and anti-inflammatory activity proved that synthesized Au nanoparticles is good alternatives in medicine and industrial application.