Ovarian Microenvironment Modulation by Adipose-Mesenchymal Stem Cells and Photobiomodulation Can Alter Osteoblasts Functions In Vitro
Purpose In tissue engineering and regenerative medicine, stem cells are used to produce tissues and organs for both physiological and clinical applications. The interactions among different organs and systems are controlled by chemical and physical stimuli, such as those induced by mesenchymal stem...
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| Veröffentlicht in: | Regenerative engineering and translational medicine 2023-12, Vol.9 (4), p.506-517 |
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| creator | Alves, E. D. Benevenuto, L. G. D. Morais, B. P. Barros, M. A. Achcar, J. A. Montrezor, L. H. |
| description | Purpose
In tissue engineering and regenerative medicine, stem cells are used to produce tissues and organs for both physiological and clinical applications. The interactions among different organs and systems are controlled by chemical and physical stimuli, such as those induced by mesenchymal stem cells (MSC) and photobiomodulation (PBM), respectively. The aim of the present study was to investigate the effects of adipose-derived MSC (AdMSC) and PBM modulations in adult female rat ovaries induced to polycystic ovary syndrome (PCOS), in terms of the number of bone marrow stromal cells obtained and the differentiation of these cells into osteoblasts during 14 days of in vitro culture.
Methods
Bone marrow cells were cultured for 14 days, in the absence and presence of testosterone, prior to analysis of different cellular parameters. The data were analyzed using ANOVA and Fisher’s tests, with statistically significant differences among the means considered for
p
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| doi_str_mv | 10.1007/s40883-023-00297-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2898433528</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2898433528</sourcerecordid><originalsourceid>FETCH-LOGICAL-c270t-6c416a445f6a164c4bc090d1c19a54db6a1cd5e64dd2dc85ac30ae337222d32e3</originalsourceid><addsrcrecordid>eNp9kNtKAzEQhoMoWLQv4FXA69Wc9nRZFquFlgoebkM2Se2W3aQm2cI-gO9t6oq982KYYeb__4EPgBuM7jBC-b1nqChogkgsRMo8Gc7AhNCMJQwzfP43U3oJpt7vEEK4IFlWpBPwtT4I1wgDV410VptD46zptAlwZVXfitBYA-sBzlSzt14nK-21kduhEy18CbqDlW5bD4VR8Hlrg60b252MVQyetUE7uPZB27oVPng47408nj1cGPjeBGevwcVGtF5Pf_sVeJs_vFZPyXL9uKhmy0SSHIUkkwxngrF0kwmcMclqiUqksMSlSJmq41aqVGdMKaJkkQpJkdCU5oQQRYmmV-B2zN07-9lrH_jO9s7El5wUZcEoTUkRVWRURSLeO73he9d0wg0cI34kzkfiPBLnP8T5EE10NPkoNh_anaL_cX0DhyWG2Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2898433528</pqid></control><display><type>article</type><title>Ovarian Microenvironment Modulation by Adipose-Mesenchymal Stem Cells and Photobiomodulation Can Alter Osteoblasts Functions In Vitro</title><source>SpringerLink Journals - AutoHoldings</source><creator>Alves, E. D. ; Benevenuto, L. G. D. ; Morais, B. P. ; Barros, M. A. ; Achcar, J. A. ; Montrezor, L. H.</creator><creatorcontrib>Alves, E. D. ; Benevenuto, L. G. D. ; Morais, B. P. ; Barros, M. A. ; Achcar, J. A. ; Montrezor, L. H.</creatorcontrib><description>Purpose
In tissue engineering and regenerative medicine, stem cells are used to produce tissues and organs for both physiological and clinical applications. The interactions among different organs and systems are controlled by chemical and physical stimuli, such as those induced by mesenchymal stem cells (MSC) and photobiomodulation (PBM), respectively. The aim of the present study was to investigate the effects of adipose-derived MSC (AdMSC) and PBM modulations in adult female rat ovaries induced to polycystic ovary syndrome (PCOS), in terms of the number of bone marrow stromal cells obtained and the differentiation of these cells into osteoblasts during 14 days of in vitro culture.
Methods
Bone marrow cells were cultured for 14 days, in the absence and presence of testosterone, prior to analysis of different cellular parameters. The data were analyzed using ANOVA and Fisher’s tests, with statistically significant differences among the means considered for
p
< 0.05.
Results
For all groups, the highest mean cell number was obtained after 60 days of in vivo treatments. PBM for 30 days and AdMSC for 60 days positively influenced the cell number before cell differentiation. After 14 days in an osteogenic medium, cell multiplication, cell viability, mineralization, and alkaline phosphatase activity were modulated by the chemical and physical treatments, as well as by testosterone.
Conclusion
The proposed model suggests that ovarian microenvironment modulations induced by mesenchymal stem cells, photobiomodulation, and testosterone can alter the behavior of osteoblasts cultured for 14 days.
Lay Summary
Homeostasis among organs and systems is maintained by complex mechanisms that interact in precisely and continuously ways, controlled by hormones including local autocrine and paracrine signals and systemic endocrine signals. These biomolecules maintain the dynamic balance of organic microenvironments and can be modulated by mesenchymal stem cells. Physical stimuli, such as photobiomodulation, can also modulate tissue functions and the interaction among organs. Hence, knowing the cell microenvironment control mechanisms is an essential aspect of tissue engineering and regenerative medicine.</description><identifier>ISSN: 2364-4133</identifier><identifier>EISSN: 2364-4141</identifier><identifier>DOI: 10.1007/s40883-023-00297-y</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Alkaline phosphatase ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomolecules ; Bone marrow ; Chemistry and Materials Science ; Differentiation (biology) ; Homeostasis ; Hormones ; In vitro methods and tests ; In vivo methods and tests ; Materials Science ; Organs ; Original Research ; Osteoblasts ; Ovaries ; Regenerative medicine ; Regenerative Medicine/Tissue Engineering ; Stem cells ; Stimuli ; Testosterone ; Tissue engineering</subject><ispartof>Regenerative engineering and translational medicine, 2023-12, Vol.9 (4), p.506-517</ispartof><rights>The Author(s), under exclusive licence to The Regenerative Engineering Society 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-6c416a445f6a164c4bc090d1c19a54db6a1cd5e64dd2dc85ac30ae337222d32e3</cites><orcidid>0000-0002-2357-0263 ; 0000-0003-1022-3358 ; 0000-0002-1096-0617 ; 0000-0003-0932-475X ; 0000-0002-0533-7169 ; 0000-0002-9868-9453</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40883-023-00297-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40883-023-00297-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Alves, E. D.</creatorcontrib><creatorcontrib>Benevenuto, L. G. D.</creatorcontrib><creatorcontrib>Morais, B. P.</creatorcontrib><creatorcontrib>Barros, M. A.</creatorcontrib><creatorcontrib>Achcar, J. A.</creatorcontrib><creatorcontrib>Montrezor, L. H.</creatorcontrib><title>Ovarian Microenvironment Modulation by Adipose-Mesenchymal Stem Cells and Photobiomodulation Can Alter Osteoblasts Functions In Vitro</title><title>Regenerative engineering and translational medicine</title><addtitle>Regen. Eng. Transl. Med</addtitle><description>Purpose
In tissue engineering and regenerative medicine, stem cells are used to produce tissues and organs for both physiological and clinical applications. The interactions among different organs and systems are controlled by chemical and physical stimuli, such as those induced by mesenchymal stem cells (MSC) and photobiomodulation (PBM), respectively. The aim of the present study was to investigate the effects of adipose-derived MSC (AdMSC) and PBM modulations in adult female rat ovaries induced to polycystic ovary syndrome (PCOS), in terms of the number of bone marrow stromal cells obtained and the differentiation of these cells into osteoblasts during 14 days of in vitro culture.
Methods
Bone marrow cells were cultured for 14 days, in the absence and presence of testosterone, prior to analysis of different cellular parameters. The data were analyzed using ANOVA and Fisher’s tests, with statistically significant differences among the means considered for
p
< 0.05.
Results
For all groups, the highest mean cell number was obtained after 60 days of in vivo treatments. PBM for 30 days and AdMSC for 60 days positively influenced the cell number before cell differentiation. After 14 days in an osteogenic medium, cell multiplication, cell viability, mineralization, and alkaline phosphatase activity were modulated by the chemical and physical treatments, as well as by testosterone.
Conclusion
The proposed model suggests that ovarian microenvironment modulations induced by mesenchymal stem cells, photobiomodulation, and testosterone can alter the behavior of osteoblasts cultured for 14 days.
Lay Summary
Homeostasis among organs and systems is maintained by complex mechanisms that interact in precisely and continuously ways, controlled by hormones including local autocrine and paracrine signals and systemic endocrine signals. These biomolecules maintain the dynamic balance of organic microenvironments and can be modulated by mesenchymal stem cells. Physical stimuli, such as photobiomodulation, can also modulate tissue functions and the interaction among organs. Hence, knowing the cell microenvironment control mechanisms is an essential aspect of tissue engineering and regenerative medicine.</description><subject>Alkaline phosphatase</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomolecules</subject><subject>Bone marrow</subject><subject>Chemistry and Materials Science</subject><subject>Differentiation (biology)</subject><subject>Homeostasis</subject><subject>Hormones</subject><subject>In vitro methods and tests</subject><subject>In vivo methods and tests</subject><subject>Materials Science</subject><subject>Organs</subject><subject>Original Research</subject><subject>Osteoblasts</subject><subject>Ovaries</subject><subject>Regenerative medicine</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Stem cells</subject><subject>Stimuli</subject><subject>Testosterone</subject><subject>Tissue engineering</subject><issn>2364-4133</issn><issn>2364-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kNtKAzEQhoMoWLQv4FXA69Wc9nRZFquFlgoebkM2Se2W3aQm2cI-gO9t6oq982KYYeb__4EPgBuM7jBC-b1nqChogkgsRMo8Gc7AhNCMJQwzfP43U3oJpt7vEEK4IFlWpBPwtT4I1wgDV410VptD46zptAlwZVXfitBYA-sBzlSzt14nK-21kduhEy18CbqDlW5bD4VR8Hlrg60b252MVQyetUE7uPZB27oVPng47408nj1cGPjeBGevwcVGtF5Pf_sVeJs_vFZPyXL9uKhmy0SSHIUkkwxngrF0kwmcMclqiUqksMSlSJmq41aqVGdMKaJkkQpJkdCU5oQQRYmmV-B2zN07-9lrH_jO9s7El5wUZcEoTUkRVWRURSLeO73he9d0wg0cI34kzkfiPBLnP8T5EE10NPkoNh_anaL_cX0DhyWG2Q</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Alves, E. D.</creator><creator>Benevenuto, L. G. D.</creator><creator>Morais, B. P.</creator><creator>Barros, M. A.</creator><creator>Achcar, J. A.</creator><creator>Montrezor, L. H.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-2357-0263</orcidid><orcidid>https://orcid.org/0000-0003-1022-3358</orcidid><orcidid>https://orcid.org/0000-0002-1096-0617</orcidid><orcidid>https://orcid.org/0000-0003-0932-475X</orcidid><orcidid>https://orcid.org/0000-0002-0533-7169</orcidid><orcidid>https://orcid.org/0000-0002-9868-9453</orcidid></search><sort><creationdate>20231201</creationdate><title>Ovarian Microenvironment Modulation by Adipose-Mesenchymal Stem Cells and Photobiomodulation Can Alter Osteoblasts Functions In Vitro</title><author>Alves, E. D. ; Benevenuto, L. G. D. ; Morais, B. P. ; Barros, M. A. ; Achcar, J. A. ; Montrezor, L. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-6c416a445f6a164c4bc090d1c19a54db6a1cd5e64dd2dc85ac30ae337222d32e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alkaline phosphatase</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomolecules</topic><topic>Bone marrow</topic><topic>Chemistry and Materials Science</topic><topic>Differentiation (biology)</topic><topic>Homeostasis</topic><topic>Hormones</topic><topic>In vitro methods and tests</topic><topic>In vivo methods and tests</topic><topic>Materials Science</topic><topic>Organs</topic><topic>Original Research</topic><topic>Osteoblasts</topic><topic>Ovaries</topic><topic>Regenerative medicine</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Stem cells</topic><topic>Stimuli</topic><topic>Testosterone</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alves, E. D.</creatorcontrib><creatorcontrib>Benevenuto, L. G. D.</creatorcontrib><creatorcontrib>Morais, B. P.</creatorcontrib><creatorcontrib>Barros, M. A.</creatorcontrib><creatorcontrib>Achcar, J. A.</creatorcontrib><creatorcontrib>Montrezor, L. H.</creatorcontrib><collection>CrossRef</collection><jtitle>Regenerative engineering and translational medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alves, E. D.</au><au>Benevenuto, L. G. D.</au><au>Morais, B. P.</au><au>Barros, M. A.</au><au>Achcar, J. A.</au><au>Montrezor, L. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ovarian Microenvironment Modulation by Adipose-Mesenchymal Stem Cells and Photobiomodulation Can Alter Osteoblasts Functions In Vitro</atitle><jtitle>Regenerative engineering and translational medicine</jtitle><stitle>Regen. Eng. Transl. Med</stitle><date>2023-12-01</date><risdate>2023</risdate><volume>9</volume><issue>4</issue><spage>506</spage><epage>517</epage><pages>506-517</pages><issn>2364-4133</issn><eissn>2364-4141</eissn><abstract>Purpose
In tissue engineering and regenerative medicine, stem cells are used to produce tissues and organs for both physiological and clinical applications. The interactions among different organs and systems are controlled by chemical and physical stimuli, such as those induced by mesenchymal stem cells (MSC) and photobiomodulation (PBM), respectively. The aim of the present study was to investigate the effects of adipose-derived MSC (AdMSC) and PBM modulations in adult female rat ovaries induced to polycystic ovary syndrome (PCOS), in terms of the number of bone marrow stromal cells obtained and the differentiation of these cells into osteoblasts during 14 days of in vitro culture.
Methods
Bone marrow cells were cultured for 14 days, in the absence and presence of testosterone, prior to analysis of different cellular parameters. The data were analyzed using ANOVA and Fisher’s tests, with statistically significant differences among the means considered for
p
< 0.05.
Results
For all groups, the highest mean cell number was obtained after 60 days of in vivo treatments. PBM for 30 days and AdMSC for 60 days positively influenced the cell number before cell differentiation. After 14 days in an osteogenic medium, cell multiplication, cell viability, mineralization, and alkaline phosphatase activity were modulated by the chemical and physical treatments, as well as by testosterone.
Conclusion
The proposed model suggests that ovarian microenvironment modulations induced by mesenchymal stem cells, photobiomodulation, and testosterone can alter the behavior of osteoblasts cultured for 14 days.
Lay Summary
Homeostasis among organs and systems is maintained by complex mechanisms that interact in precisely and continuously ways, controlled by hormones including local autocrine and paracrine signals and systemic endocrine signals. These biomolecules maintain the dynamic balance of organic microenvironments and can be modulated by mesenchymal stem cells. Physical stimuli, such as photobiomodulation, can also modulate tissue functions and the interaction among organs. Hence, knowing the cell microenvironment control mechanisms is an essential aspect of tissue engineering and regenerative medicine.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s40883-023-00297-y</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-2357-0263</orcidid><orcidid>https://orcid.org/0000-0003-1022-3358</orcidid><orcidid>https://orcid.org/0000-0002-1096-0617</orcidid><orcidid>https://orcid.org/0000-0003-0932-475X</orcidid><orcidid>https://orcid.org/0000-0002-0533-7169</orcidid><orcidid>https://orcid.org/0000-0002-9868-9453</orcidid></addata></record> |
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| subjects | Alkaline phosphatase Biomaterials Biomedical Engineering and Bioengineering Biomolecules Bone marrow Chemistry and Materials Science Differentiation (biology) Homeostasis Hormones In vitro methods and tests In vivo methods and tests Materials Science Organs Original Research Osteoblasts Ovaries Regenerative medicine Regenerative Medicine/Tissue Engineering Stem cells Stimuli Testosterone Tissue engineering |
| title | Ovarian Microenvironment Modulation by Adipose-Mesenchymal Stem Cells and Photobiomodulation Can Alter Osteoblasts Functions In Vitro |
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